[Histonet] Histogel

[Peter Carroll]

 > histogrl backlash

thats a whole 'nother set of issues altogether ;)

happy friday!



anh2006 <@t> med.cornell.edu wrote:
> Wow, what is the histogrl backlash? Will someone please summarize the issues people are having?
>
> Thanks!
> Andrea
>
> -----Original Message-----
> From: "Hofecker, Jennifer L" <jennifer.l.hofecker <@t> Vanderbilt.Edu>
>
> Date: Fri, 20 Mar 2009 08:23:02 
> To: <histonet <@t> lists.utsouthwestern.edu>; Andrea Grantham<algranth <@t> u.arizona.edu>
> Subject: RE: [Histonet] processing v-e-r-y tiny samples
>
>
> Hi Andi, Happy Friday!
> I know there's been a "Histogel backlash" of late, but I would still
> recommend trying it. I haven't had any problems. We use it for scant
> neuro specimens every day. We also have used H.G. for mouse sural nerve
> biopsies that needed to retain orientation (very tiny). If you do use
> Histogel, do not put it in a histoscreen or biopsy cassette and be sure
> to put it on a process that's long enough. People often use the
> cassette/process based on the size of the tissue, not of the Histogel
> block. I'd ask thermo for a sample of H.G. and try it. If you need any
> Histogel help, let me know. I'm a huge fan!
> Have a great day,
> Jennifer 
>
> Jennifer L. Hofecker HT(ASCP)
> Vanderbilt University Medical Center
> Division of Neuropathology
> Nashville, TN 
> ph 615.343.0083
> fax 615.343.7089
> -----Original Message-----
> From: Bryan Watson [mailto:Bryan.Watson <@t> parkview.com] 
> Sent: Thursday, March 19, 2009 3:39 PM
> To: histonet <@t> lists.utsouthwestern.edu; Andrea Grantham
> Subject: Re: [Histonet] processing v-e-r-y tiny samples
>
> I may never complain about tiny GI or bronch biopsies ever again!
>
>   
>>>> Andrea Grantham <algranth <@t> u.arizona.edu> 3/19/2009 11:51 AM >>>
>>>>         
> Good Morning!
> In keeping with the weirdness of the projects I get in this lab today 
> my question is about processing mosquito GI tracts.
> I have a processing schedule - that is not the problem. I'm wondering 
> if anybody out in histoland has a suggestion for what kind of 
> cassette to use. I was thinking of the histoscreen cassette because 
> these GI tracts are so thin (I think thinner than a hair)and I don't 
> want to wrap them or use sponges because I'm afraid that I'll loose 
> them or crush them.
> Any ideas?
>
> Andi
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044                     :
> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> : (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
> :...................................................................:
>            http://www.cba.arizona.edu/histology-lab.html 
>
>
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