[Histonet] processing v-e-r-y tiny samples

[Hofecker, Jennifer L]

Hi Andi, Happy Friday!
I know there's been a "Histogel backlash" of late, but I would still
recommend trying it. I haven't had any problems. We use it for scant
neuro specimens every day. We also have used H.G. for mouse sural nerve
biopsies that needed to retain orientation (very tiny). If you do use
Histogel, do not put it in a histoscreen or biopsy cassette and be sure
to put it on a process that's long enough. People often use the
cassette/process based on the size of the tissue, not of the Histogel
block. I'd ask thermo for a sample of H.G. and try it. If you need any
Histogel help, let me know. I'm a huge fan!
Have a great day,
Jennifer 

Jennifer L. Hofecker HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
Nashville, TN 
ph 615.343.0083
fax 615.343.7089
-----Original Message-----
From: Bryan Watson [mailto:Bryan.Watson <@t> parkview.com] 
Sent: Thursday, March 19, 2009 3:39 PM
To: histonet <@t> lists.utsouthwestern.edu; Andrea Grantham
Subject: Re: [Histonet] processing v-e-r-y tiny samples

I may never complain about tiny GI or bronch biopsies ever again!

>>> Andrea Grantham <algranth <@t> u.arizona.edu> 3/19/2009 11:51 AM >>>
Good Morning!
In keeping with the weirdness of the projects I get in this lab today 
my question is about processing mosquito GI tracts.
I have a processing schedule - that is not the problem. I'm wondering 
if anybody out in histoland has a suggestion for what kind of 
cassette to use. I was thinking of the histoscreen cassette because 
these GI tracts are so thin (I think thinner than a hair)and I don't 
want to wrap them or use sponges because I'm afraid that I'll loose 
them or crush them.
Any ideas?

Andi
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
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