[Histonet] processing v-e-r-y tiny samples

[louise renton]

Shandon - or whatever they're called this week- sell nylon mesh biopsy bags.
These are flat bags, sealed on 3 sides. I have found that if you cut one
corner off (ie, a oblong  piece with 2 sides still  sealed) just a tiny bit
bigger than the cassette, you can open the bag, like a book, insert the
sample and flatten the bag over the specimen and place it snug and flat in
the bottom of the casette. It sems to stay like that through processing (esp
if kept the cassette is kept flat in the basket), and is easy to locate
against the mesh for embedding.

On 3/19/09, Andrea Grantham <algranth <@t> u.arizona.edu> wrote:
>
> Good Morning!
> In keeping with the weirdness of the projects I get in this lab today my
> question is about processing mosquito GI tracts.
> I have a processing schedule - that is not the problem. I'm wondering if
> anybody out in histoland has a suggestion for what kind of cassette to use.
> I was thinking of the histoscreen cassette because these GI tracts are so
> thin (I think thinner than a hair)and I don't want to wrap them or use
> sponges because I'm afraid that I'll loose them or crush them.
> Any ideas?
>
> Andi
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
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