[Histonet] RE: Paraffin Block Trimming
Serafin Cristina
serafin <@t> HHSC.CA
Thu Mar 19 13:02:51 CDT 2009
We use a paraffin block dewaxer in our lab. It works great. I find it saves time and doesn't create as much of a mess (with wax shavings). Some people in our lab still prefer to trim the excess wax with a blade or forceps. I guess it's all personal preference.
----------------------------------------------------------------------
Message: 1
Date: Thu, 19 Mar 2009 15:16:22 +0000
From: anh2006 <@t> med.cornell.edu
Subject: Re: [Histonet] Paraffin Block trimming
To: "Merced Leiker" <leiker <@t> buffalo.edu>, "Martin, Gary"
<gmartin <@t> marshallmedical.org>, "Bartlett, Jeanine (CDC/CCID/NCZVED)"
<jqb7 <@t> cdc.gov>, "Paula Pierce" <contact <@t> excaliburpathology.com>,
"Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<793471102-1237476050-cardhu_decombobulator_blackberry.rim.net-1735884627- <@t> bxe1028.bisx.prod.on.blackberry>
Content-Type: text/plain; charset="Windows-1252"
I don't normally embed or cut my own sections anymore, but when I was doing it a few years ago on a larger scale (but still in a research setting), clean embedding generally did the trick.
But when I did need to remove the excess wax on the sides of the cassette, I would melt it using a hotplate.
In fact I nearly always did this during the embedding process. Once the blocks had solidified I would go back and melt the wax on the sides using the hot plate of the embedding station.
Only if needed, on an individual block basis, would I scrape with a straight edge blade at the time of sectioning.
-----Original Message-----
From: Merced Leiker <leiker <@t> buffalo.edu>
Date: Thu, 19 Mar 2009 10:55:36
To: Martin, Gary<gmartin <@t> marshallmedical.org>; Bartlett, Jeanine (CDC/CCID/NCZVED)<jqb7 <@t> cdc.gov>; Paula Pierce<contact <@t> excaliburpathology.com>; Histonet<histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] Paraffin Block trimming
Now I'm interested in how you embed so as to not have to scrape...if i
don't add enough wax (enough to end up rising around the edges of the
cassette, hence the scraping later), i don't get a secure hold of the block
to the cassette...
ML
--On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary"
<gmartin <@t> marshallmedical.org> wrote:
> No you're not the only one ... I was wondering the same thing ... why all
> the scraping. It seems to me that clean embedding does the trick with a
> few exceptions. G
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
> Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM
> To: Paula Pierce; Histonet
> Subject: RE: [Histonet] Paraffin Block triming
>
> I was wondering if I was the only one out there that rarely has to scrape
> a block.
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
> Pierce Sent: Thursday, March 19, 2009 9:57 AM
> To: Histonet
> Subject: [Histonet] Paraffin Block triming
>
> I try to embed so as to have a minimal amount of paraffin to scrape from
> the blocks. ;)
>
> But, I do scrape using the handle end of the same forceps I use to pick
> up the ribbon and tease the sections. No sharp edge. No electricity.
>
> PKP
>
>
>
>
>
>
>________________________________
> From: Peter Carroll <carrolpb <@t> umdnj.edu>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Thursday, March 19, 2009 8:30:34 AM
> Subject: Re: [Histonet] Paraffin Block triming
>
>> Does anybody use a paraffin block dewaxer ?
>
> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find
> that it's not only very quick, but quite accurate...
>
>
>
> Scott wrote:
>> Hi,
>> Does anybody use a paraffin block dewaxer ? If so does it save any
>> time, how well does it work?
>>
>> Thanks,
>>
>> Scott Hendricksen HT (ASCP)
>>_______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>
>
>_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Hi,
> Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 19 Mar 2009 08:45:23 -0700
From: "Jennifer Campbell" <jcampbell <@t> vdxpathology.com>
Subject: [Histonet] dako autostainers
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5658CBDB9EAE6545ABE50D2563D81BF81DF4FA <@t> VDXSERVER01.vdxpathology.local>
Content-Type: text/plain; charset="us-ascii"
We are looking into buying the Dako Autostainer Plus. I asked our rep
if they still have any older models around to look into. He made it
sound like there are older models but, the stainer itself has stayed the
same, just the software has changed/improved. He said they don't even
like selling the older models anymore b/c they are worried that the
software would be out of date. I looked into this on Dako.com and found
that there is the older Dado Autostainer. After reading through its
description it looks like it just lacks a few of the bells and whistles
(like a slide labeler for example) that the Autostainer Plus has but,
otherwise looks like it gets the job done just the same. Does anyone
have any feedback on either one of these machines and whether or not you
think I should even bother looking into the older model? Thank you so
much.
Jen C.
------------------------------
Message: 6
Date: Thu, 19 Mar 2009 11:45:28 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Paraffin Block trimming
To: "Merced Leiker" <leiker <@t> buffalo.edu>, "Martin, Gary"
<gmartin <@t> marshallmedical.org>, "Paula Pierce"
<contact <@t> excaliburpathology.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9A16CB5D55FC1648ADF11B63E72A1BE1BF03E7 <@t> LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=iso-8859-1
We have used a variety of cassettes and paraffin over the years...same result.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: Merced Leiker [mailto:leiker <@t> buffalo.edu]
Sent: Thursday, March 19, 2009 11:16 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block trimming
hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes)
--On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine
(CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov> wrote:
> Don't know what to say.........I fill mine up and as long as I don't get
> to rough with it (bump it or something where it sloshes) I don't have any
> excess paraffin around the edges. I use metal base molds.
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: Merced Leiker [mailto:leiker <@t> buffalo.edu]
> Sent: Thursday, March 19, 2009 10:56 AM
> To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce;
> Histonet Subject: RE: [Histonet] Paraffin Block trimming
>
> Now I'm interested in how you embed so as to not have to scrape...if i
> don't add enough wax (enough to end up rising around the edges of the
> cassette, hence the scraping later), i don't get a secure hold of the
> block to the cassette...
>
> ML
>
> --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary"
> <gmartin <@t> marshallmedical.org> wrote:
>
>> No you're not the only one ... I was wondering the same thing ... why all
>> the scraping. It seems to me that clean embedding does the trick with a
>> few exceptions. G
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
>> Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM
>> To: Paula Pierce; Histonet
>> Subject: RE: [Histonet] Paraffin Block triming
>>
>> I was wondering if I was the only one out there that rarely has to scrape
>> a block.
>>
>>
>> Jeanine Bartlett
>> Infectious Diseases Pathology Branch
>> (404) 639-3590
>> jeanine.bartlett <@t> cdc.hhs.gov
>>
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
>> Pierce Sent: Thursday, March 19, 2009 9:57 AM
>> To: Histonet
>> Subject: [Histonet] Paraffin Block triming
>>
>> I try to embed so as to have a minimal amount of paraffin to scrape from
>> the blocks. ;)
>>
>> But, I do scrape using the handle end of the same forceps I use to pick
>> up the ribbon and tease the sections. No sharp edge. No electricity.
>>
>> PKP
>>
>>
>>
>>
>>
>>
>> ________________________________
>> From: Peter Carroll <carrolpb <@t> umdnj.edu>
>> To: histonet <@t> lists.utsouthwestern.edu
>> Sent: Thursday, March 19, 2009 8:30:34 AM
>> Subject: Re: [Histonet] Paraffin Block triming
>>
>>> Does anybody use a paraffin block dewaxer ?
>>
>> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find
>> that it's not only very quick, but quite accurate...
>>
>>
>>
>> Scott wrote:
>>> Hi,
>>> Does anybody use a paraffin block dewaxer ? If so does it save any
>>> time, how well does it work?
>>>
>>> Thanks,
>>>
>>> Scott Hendricksen HT (ASCP)
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>>
>>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> Merced M Leiker
> Research Technician II
> 354 Biomedical Research Building
> School of Medicine and Biomedical Sciences
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214
> Ph: (716) 829-6033
> Fx: (716) 829-2725
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
>
>
Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
------------------------------
Message: 7
Date: Thu, 19 Mar 2009 08:51:01 -0700
From: Andrea Grantham <algranth <@t> u.arizona.edu>
Subject: [Histonet] processing v-e-r-y tiny samples
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.2.3.4.1.20090319084302.027167d0 <@t> algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Good Morning!
In keeping with the weirdness of the projects I get in this lab today
my question is about processing mosquito GI tracts.
I have a processing schedule - that is not the problem. I'm wondering
if anybody out in histoland has a suggestion for what kind of
cassette to use. I was thinking of the histoscreen cassette because
these GI tracts are so thin (I think thinner than a hair)and I don't
want to wrap them or use sponges because I'm afraid that I'll loose
them or crush them.
Any ideas?
Andi
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
------------------------------
Message: 8
Date: Thu, 19 Mar 2009 11:56:41 -0400
From: Peter Carroll <carrolpb <@t> umdnj.edu>
Subject: Re: [Histonet] processing v-e-r-y tiny samples
To: Andrea Grantham <algranth <@t> u.arizona.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49C26B39.8020502 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
i use those tiny mesh inserts for small biopsies, or in a pinch, i just
wrap the tissue in a square of lint-free lens-cleaning paper...
Andrea Grantham wrote:
> Good Morning!
> In keeping with the weirdness of the projects I get in this lab today
> my question is about processing mosquito GI tracts.
> I have a processing schedule - that is not the problem. I'm wondering
> if anybody out in histoland has a suggestion for what kind of cassette
> to use. I was thinking of the histoscreen cassette because these GI
> tracts are so thin (I think thinner than a hair)and I don't want to
> wrap them or use sponges because I'm afraid that I'll loose them or
> crush them.
> Any ideas?
>
> Andi
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 9
Date: Thu, 19 Mar 2009 09:02:50 -0700
From: "Shakun Aswani" <Shakun.Aswani <@t> acologix.com>
Subject: RE: [Histonet] Paraffin Block triming
To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>, "Paula
Pierce" <contact <@t> excaliburpathology.com>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<777AB0DE519C8E46A6220E2287C5BAD301ADE586 <@t> EXCHANGE.acologix.com>
Content-Type: text/plain; charset="iso-8859-1"
Jeanine,
We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys
Shakun
-----Original Message-----
From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7 <@t> cdc.gov]
Sent: Thursday, March 19, 2009 8:45 AM
To: Shakun Aswani; Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming
Of course Shakun....you trained me!
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: Shakun Aswani [mailto:Shakun.Aswani <@t> acologix.com]
Sent: Thursday, March 19, 2009 11:09 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming
I am also one of that
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, March 19, 2009 6:58 AM
To: Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming
I was wondering if I was the only one out there that rarely has to scrape a block.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, March 19, 2009 9:57 AM
To: Histonet
Subject: [Histonet] Paraffin Block triming
I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;)
But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity.
PKP
________________________________
From: Peter Carroll <carrolpb <@t> umdnj.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 8:30:34 AM
Subject: Re: [Histonet] Paraffin Block triming
> Does anybody use a paraffin block dewaxer ?
Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate...
Scott wrote:
> Hi,
> Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 19 Mar 2009 12:06:24 -0400
From: Merced Leiker <leiker <@t> buffalo.edu>
Subject: RE: [Histonet] Paraffin Block trimming
To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>, "Martin,
Gary" <gmartin <@t> marshallmedical.org>, Paula Pierce
<contact <@t> excaliburpathology.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4BD853A8A206B35B3566FC91 <@t> bchwxp2702.ad.med.buffalo.edu>
Content-Type: text/plain; charset=iso-8859-1; format=flowed
I guess one has to go to histology school to learn how to do it, then (sent
under separate cover from another histonetter...) I 'm too researchy. ;-)
--On Thursday, March 19, 2009 11:45 AM -0400 "Bartlett, Jeanine
(CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov> wrote:
> We have used a variety of cassettes and paraffin over the years...same
> result.
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: Merced Leiker [mailto:leiker <@t> buffalo.edu]
> Sent: Thursday, March 19, 2009 11:16 AM
> To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce;
> Histonet Subject: RE: [Histonet] Paraffin Block trimming
>
> hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base
> Molds...maybe it's the cassette design or type of wax used? (VWR
> 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics
> Biopsy Cassettes)
>
>
> --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine
> (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov> wrote:
>
>> Don't know what to say.........I fill mine up and as long as I don't get
>> to rough with it (bump it or something where it sloshes) I don't have any
>> excess paraffin around the edges. I use metal base molds.
>>
>>
>> Jeanine Bartlett
>> Infectious Diseases Pathology Branch
>> (404) 639-3590
>> jeanine.bartlett <@t> cdc.hhs.gov
>>
>>
>> -----Original Message-----
>> From: Merced Leiker [mailto:leiker <@t> buffalo.edu]
>> Sent: Thursday, March 19, 2009 10:56 AM
>> To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce;
>> Histonet Subject: RE: [Histonet] Paraffin Block trimming
>>
>> Now I'm interested in how you embed so as to not have to scrape...if i
>> don't add enough wax (enough to end up rising around the edges of the
>> cassette, hence the scraping later), i don't get a secure hold of the
>> block to the cassette...
>>
>> ML
>>
>> --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary"
>> <gmartin <@t> marshallmedical.org> wrote:
>>
>>> No you're not the only one ... I was wondering the same thing ... why
>>> all the scraping. It seems to me that clean embedding does the trick
>>> with a few exceptions. G
>>>
>>> -----Original Message-----
>>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>>> Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58
>>> AM To: Paula Pierce; Histonet
>>> Subject: RE: [Histonet] Paraffin Block triming
>>>
>>> I was wondering if I was the only one out there that rarely has to
>>> scrape a block.
>>>
>>>
>>> Jeanine Bartlett
>>> Infectious Diseases Pathology Branch
>>> (404) 639-3590
>>> jeanine.bartlett <@t> cdc.hhs.gov
>>>
>>>
>>> -----Original Message-----
>>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
>>> Pierce Sent: Thursday, March 19, 2009 9:57 AM
>>> To: Histonet
>>> Subject: [Histonet] Paraffin Block triming
>>>
>>> I try to embed so as to have a minimal amount of paraffin to scrape from
>>> the blocks. ;)
>>>
>>> But, I do scrape using the handle end of the same forceps I use to pick
>>> up the ribbon and tease the sections. No sharp edge. No electricity.
>>>
>>> PKP
>>>
>>>
>>>
>>>
>>>
>>>
>>> ________________________________
>>> From: Peter Carroll <carrolpb <@t> umdnj.edu>
>>> To: histonet <@t> lists.utsouthwestern.edu
>>> Sent: Thursday, March 19, 2009 8:30:34 AM
>>> Subject: Re: [Histonet] Paraffin Block triming
>>>
>>>> Does anybody use a paraffin block dewaxer ?
>>>
>>> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find
>>> that it's not only very quick, but quite accurate...
>>>
>>>
>>>
>>> Scott wrote:
>>>> Hi,
>>>> Does anybody use a paraffin block dewaxer ? If so does it save any
>>>> time, how well does it work?
>>>>
>>>> Thanks,
>>>>
>>>> Scott Hendricksen HT (ASCP)
>>>> _______________________________________________
>>>> Histonet mailing list
>>>> Histonet <@t> lists.utsouthwestern.edu
>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>>
>>>>
>>>>
>>>
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>>
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>
>>
>>
>> Merced M Leiker
>> Research Technician II
>> 354 Biomedical Research Building
>> School of Medicine and Biomedical Sciences
>> State University of New York at Buffalo
>> 3435 Main St, Buffalo, NY 14214
>> Ph: (716) 829-6033
>> Fx: (716) 829-2725
>>
>> No trees were harmed in the sending of this email.
>> However, many electrons were severely inconvenienced.
>>
>>
>>
>>
>
>
>
> Merced M Leiker
> Research Technician II
> 354 Biomedical Research Building
> School of Medicine and Biomedical Sciences
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214
> Ph: (716) 829-6033
> Fx: (716) 829-2725
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
>
>
Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
------------------------------
Message: 11
Date: Thu, 19 Mar 2009 09:15:57 -0700
From: Andrea Grantham <algranth <@t> u.arizona.edu>
Subject: Re: [Histonet] processing v-e-r-y tiny samples
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.2.3.4.1.20090319091350.02723530 <@t> algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Jeff - This is not an early April Fools joke.
I don't want to wrap them so that they don't get really flattened out.
I'm thinking to put them in histogel or agar now to make the
orientation easier when embedding. Good idea?
Andi
At 08:51 AM 3/19/2009, Andrea Grantham wrote:
>Good Morning!
>In keeping with the weirdness of the projects I get in this lab
>today my question is about processing mosquito GI tracts.
>I have a processing schedule - that is not the problem. I'm
>wondering if anybody out in histoland has a suggestion for what kind
>of cassette to use. I was thinking of the histoscreen cassette
>because these GI tracts are so thin (I think thinner than a hair)and
>I don't want to wrap them or use sponges because I'm afraid that
>I'll loose them or crush them.
>Any ideas?
>
>Andi
>.....................................................................
>: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
>: Sr. Research Specialist University of Arizona :
>: (office: AHSC 4212) P.O. Box 245044 :
>: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
>: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
>:...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
------------------------------
Message: 12
Date: Thu, 19 Mar 2009 09:14:56 -0700
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: [Histonet] Xmatrx (Rx)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bdfbc2370903190914y1c4023e8j8e9066c366e57db <@t> mail.gmail.com>
Content-Type: text/plain; charset=windows-1252
Histonet,
Has anyone heard of this machine? We recently had a BioGenex sales person
come and introduce it to us. I never knew that it existed, I've primarily
used biogenex for antibodies. The literature looks interesting, it
potentially can do IHC, CISH, SS, FISH, TUNEL on FFPE, Cellular prep, cDNA
microarrays, PCR etc. It almost looks like an open version of the Ventana,
with a couple of differences. Are there any labs using it? Any that would
be willing to share their impressions?
Thanks for your time
--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com
------------------------------
Message: 13
Date: Thu, 19 Mar 2009 09:25:12 -0700
From: Andrea Grantham <algranth <@t> u.arizona.edu>
Subject: [Histonet] PSLIM slide printer
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.2.3.4.1.20090319091718.02751970 <@t> algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I think mine was probably the last lab to purchase a PSLIM from
AccuPlace before they sold off the printer to Fisher. Just in time!
For what it is worth, I do like the printer. It is great for a small
lab and is saving what is left of my wrist. However, we are on the
second machine. The first one wasted about as many slides as I
accepted. We called tech support and tried their suggestions but
nothing worked. After we sent them a picture of what the slides
looked like they called with an RMA number and sent out a replacement
that is working perfectly.
I'm happy!
We had an option of purchasing a service/replacement agreement with
the printer and I'm glad we did. I don't know if Fisher is offering
this option but if you are considering the printer it might be a good idea.
Andi
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
------------------------------
Message: 14
Date: Thu, 19 Mar 2009 11:33:14 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: [Histonet] Seeking reference lab for Langerin glycoprotein
IHC staining
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
<5998F3BDFF7AAC4091C7AE93A7A1A5892CDF1C <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
One of our pathologists is looking for a reference lab to perform
Langerin Glycoprotein IHC staining on FFPE human tissue.
Any and all responses are welcome.
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
------------------------------
Message: 15
Date: Thu, 19 Mar 2009 11:37:04 -0500
From: "Doug Showers" <Doug.Showers <@t> propath.com>
Subject: RE: [Histonet] Paraffin Block triming
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<82C7248978CB50469FD6BA68EBBEFE67EB477E <@t> exchange.propathlab.com>
Content-Type: text/plain; charset="iso-8859-1"
I think the amount of excess paraffin depends on the temperature of the mold
when you are embedding. I like my molds "cold" at room temperature and don't
have much leakage whereas techs who keep the molds "hot" have a higher amount
of leakage around the edges.
Doug Showers, MS, HT
Histology Manager
ProPath
8267 Elmbrook Dr. Suite 100
Dallas, TX 75247
To learn more about ProPath, please visit http://www.ProPathLab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shakun Aswani
Sent: Thursday, March 19, 2009 11:03 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming
Jeanine,
We had the best Chief instructor Billie Swisher. I am so very thankful that
I got the training from that school. I do miss you guys
Shakun
-----Original Message-----
From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7 <@t> cdc.gov]
Sent: Thursday, March 19, 2009 8:45 AM
To: Shakun Aswani; Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming
Of course Shakun....you trained me!
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: Shakun Aswani [mailto:Shakun.Aswani <@t> acologix.com]
Sent: Thursday, March 19, 2009 11:09 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming
I am also one of that
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, March 19, 2009 6:58 AM
To: Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming
I was wondering if I was the only one out there that rarely has to scrape a
block.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, March 19, 2009 9:57 AM
To: Histonet
Subject: [Histonet] Paraffin Block triming
I try to embed so as to have a minimal amount of paraffin to scrape from the
blocks. ;)
But, I do scrape using the handle end of the same forceps I use to pick up
the ribbon and tease the sections. No sharp edge. No electricity.
PKP
________________________________
From: Peter Carroll <carrolpb <@t> umdnj.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 8:30:34 AM
Subject: Re: [Histonet] Paraffin Block triming
> Does anybody use a paraffin block dewaxer ?
Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that
it's not only very quick, but quite accurate...
Scott wrote:
> Hi,
> Does anybody use a paraffin block dewaxer ? If so does it save any
time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
This information is directed in confidence solely to the person
named above and may not otherwise be distributed, copied or
disclosed. Therefore, this information should be considered
strictly confidential. If you have received this email in error, please
notify the sender immediately via a return email for further
direction. Thank you for your assistance.
More information about the Histonet
mailing list