[Histonet] Paraffin Block trimming

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

Don't know what to say.........I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges.  I use metal base molds. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: Merced Leiker [mailto:leiker <@t> buffalo.edu] 
Sent: Thursday, March 19, 2009 10:56 AM
To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block trimming

Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette...

ML

--On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" 
<gmartin <@t> marshallmedical.org> wrote:

> No you're not the only one ... I was wondering the same thing ... why all
> the scraping.  It seems to me that clean embedding does the trick with a
> few exceptions.  G
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
> Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM
> To: Paula Pierce; Histonet
> Subject: RE: [Histonet] Paraffin Block triming
>
> I was wondering if I was the only one out there that rarely has to scrape
> a block.
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
> Pierce Sent: Thursday, March 19, 2009 9:57 AM
> To: Histonet
> Subject: [Histonet] Paraffin Block triming
>
> I try to embed so as to have a minimal amount of paraffin to scrape from
> the blocks. ;)
>
> But, I do scrape using the handle end of the same forceps I use to pick
> up the ribbon and tease the sections. No sharp edge. No electricity.
>
> PKP
>
>
>  
>
>
>
> ________________________________
> From: Peter Carroll <carrolpb <@t> umdnj.edu>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Thursday, March 19, 2009 8:30:34 AM
> Subject: Re: [Histonet] Paraffin Block triming
>
>> Does anybody use a paraffin block dewaxer ?
>
> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find
> that it's not only very quick, but quite accurate...
>
>
>
> Scott wrote:
>> Hi,
>>     Does anybody use a paraffin block dewaxer ?  If so does it save any
>> time, how well does it work?
>>
>> Thanks,
>>
>> Scott Hendricksen  HT (ASCP)
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>>
>>
>>  
>
>
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

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