[Histonet] Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues.

[Tony Henwood]

Sarah,

Skin surface tends to dry out as well as the epithelial cells lose their
nuclei as they migrate to the surface.
The surface can also dry out after the application of antiseptics etc
prior to biopsy.
AND there is often the issue of the biopsy being allowed to dry prior to
fixation.

This will affect the haematoxylin staining.

To improve the staining, try pretreating the dewaxed sections with 1%
periodic acid for 10 minutes prior to H&E staining. This might work.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of SARAH
REEVES
Sent: Tuesday, 17 March 2009 7:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Does anyone know why skin sections do not seem to
take up haematoxylin as well as other tissues. The chromatin detail is
not as good as with other tissues.


Does anyone know why skin sections do not seem to take up haematoxylin
as well as other tissues. The chromatin detail is not as good as with
other tissues.


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