[Histonet] curling on cryostat brain sections and other issues

Peter Carroll carrolpb <@t> umdnj.edu
Tue Mar 17 11:41:27 CDT 2009


er, sit for a few moments under the anti-curling plate, that is...

Peter Carroll wrote:
> > I am having  problems with my brain sections  (15 um - flash-frozen) 
> curling up once I remove the antiroll plate on my cryostat...
>
> I cut many of the same on a regular basis, and I find that letting 
> them sit for a a few moments (~10 seconds)  helps keep them from 
> curling, at least long enough to pick them up with the slide!
>
>
>>
>
>
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