[Histonet] curling on cryostat brain sections and other issues

[Guillermo Palchik]

Hi there,
I am having  problems with my brain sections  (15 um - flash-frozen)  
curling up once I remove the antiroll plate on my cryostat... does  
anyone know how i can prevent this?
Also - does anybody have a "quick guide" on what to improve while  
cutting, based on the damage seen on the brain slice: for example I  
heard that if there are vertical lines along the slice, it might mean  
a nick in the blade, that cracking of the tissue might be cutting with  
the blade too perpendicular, and so on... i am looking for a general  
troubleshooting guide for cryostat cutting...
thanks
Gil

-- 
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There are those who fight one year and are better...
There are people who fight many years and are very good...
But there are those who fight their entire lives: they are  
indispensable...

Bertolt Brecht (1898-1956)


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