[Histonet] decolorizing MMA

[Jack Ratliff]

Are you talking about thin microtomed sections or thicker "ground" sections???? Thin MMA embedded sections can be deplastified similar to the removal of paraffin embedded specimens, but thicker "ground" MMA sections cannot be deplastified.

 

However, maybe you are making reference to re-staining a slide like in a paraffin embedded H&E where you can reverse the staining order and stain again???? I have not tried it on thin sections and suspect that it would depend upon the stain used. Also, you might have a problem with the section becoming loose or falling off the slide.

 

As for the thicker "ground" sections, you should be able to re-polish the slides (depending upon true section thickness) and then stain again. Keep in mind that when staining these thicker MMA sections, most (if not all) typical stains only surface stain the section in the first place due to the size of the staining molecules and their ability to penetrate the resin w/ and/or w/o acid etching techniques.

 

Jack Ratliff

 


 
> Date: Mon, 16 Mar 2009 08:14:23 -0500
> From: BMolinari <@t> heart.thi.tmc.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] decolorizing MMA
> 
> Good morning,
> 
> Is it possible to decolorize MMA slides? 
> 
> Thanks,
> 
> Betsy
> 
> 
> 
> Betsy Molinari HT(ASCP)
> 
> Texas Heart Institute
> 
> Cardiovascular Pathology
> 
> 6770 Bertner Ave
> 
> MC1-283
> 
> Houston,TX 77030-2607
> 
> 832-355-6524
> 
> 832-355-6812
> 
> 
> 
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