[Histonet] TweakR

[Melissa Mazan]

Hi all,
I'm trying to use Tweak R (Fn14) on mouse lung.  I've got a rabbit 
polyclonal from abcam that supposedly is validated for IHC-paraffin - 
have tried it with and without HIER (citrate) and with and without 
detergents - was worried that as its a transmembrane protein maybe I was 
washing it away.  Has anybody done this on paraffins? If so, can you 
share a protocol? Many thanks - Melissa

-- 
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cummings School of Veterinary Medicine
200 Westborough Road
North Grafton,MA 01536
tel:	508-839-5395
fax:	508-839-7903
email:	melissa.mazan <@t> tufts.edu