[Histonet] IHC stainer

[Pamela Marcum]

Just be sure it is an open system that will allow you to use any primary antibody you need and secondary antibody not just a secondary kit the company provides.  You will need to confirm that if you are using a chicken primary for instance, you can get and use a secondary link that will work with the chicken primary to get your staining reaction.  Be very careful that it allows you flexibilty and is not designed for clinical standard usage with no ability to truly allow you the range required for animal work.  I work with animal and it is hard to find everyhting you need and not all "standard" antibodies and systems will work across species.  Good Luck,  Pam marcum



----- Original Message -----
From: "Greg Dobbin" <gvdobbin <@t> ihis.org>
To: histonet <@t> lists.utsouthwestern.edu, "Ronald Houston" <Ronald.Houston <@t> nationwidechildrens.org>, sweaver <@t> tvmdl.tamu.edu
Sent: Friday, March 13, 2009 8:57:48 PM GMT -05:00 US/Canada Eastern
Subject: RE: [Histonet] IHC stainer

I guess I would have to echo that as well!
Cheers!
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


>>> "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
3/13/2009 4:45:14 PM >>>
Can't speak highly enough of the BondMax form Leica Microsystems. If
cost is an issue and you're looking at what to avoid, IMHO avoid
anything Ventana

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Stephanie Weaver
Sent: Friday, March 13, 2009 3:17 PM
To: histonet post
Subject: [Histonet] IHC stainer

I am in a veterinary diagnostic lab.  In the past we have had very few
requests for IHC and have always sent slides out to another lab to
perform IHC as needed.  It is time for us to start doing our own and
join the modern age.  We have several certified technicians, but none
have experience with IHC and we typically have a relatively high
turnover rate.  Therefore, I am hoping to be able to buy an automated
stainer.  In the past most people on the list seemed to be very happy
with the Dako autostainer, but this past week has brought so many bad
remarks about Dako's service that I am reconsidering.  We probably
will
not need a high capacity autostainer, but I would like walk-away
capability with an easy to use system.  It will need to accept other
companies reagents, since veterinary infectious disease antibodies
aren't often sold by the major companies.  Also, cost is an issue and
I
would like to be able to bargain shop for reagents through other
companies.  Does anyone have any recommendations, or warnings as to
what
to avoid?

In a related issue, where do other animal tissue people get their
antibodies for infectious diseases, e.g. Parvovirus, canine distemper,
or FIP?

Thanks for the advice!




Stephanie Weaver
Texas Veterinary Medical Diagnostic Lab



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
intended recipient (or authorized to receive information for the
intended recipient), you are hereby notified that any review, use,
disclosure, distribution, copying, printing, or action taken in
reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

-------------------------
Statement of Confidentiality
This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system.

D?claration de confidentialit?
Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique.
-------------------------


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet