[Histonet] IHC stainer

[Julie Trejo]

I suggest using the Leica Bond-Max.  I, an HT(ASCP)cm, did not have any IHC
experiance, but the Bond-Max makes it happen.  Just putting dried slides on,
patient info in the computer, slides labeled and then in 3-4 hours, it's all
done.  Just dehydrate and clear.  Very simple and now giving me time to
learn IHC and troubleshooting.  Some of the antibodies come "Ready to use"
from Leica, and we titer others that aren't "Ready to use" (Biocare, Dako
etc).
   I've seen co-workers in the past struggling with immuno's using the Dako,
and taking alot of time and still counter-staining by hand.  The Bond-Max
allows us (just three of us) to do all routine H&E's, special stains,
recuts, deepers and Immuno's in one day. Not bad.

Julie
On Fri, Mar 13, 2009 at 2:16 PM, Stephanie Weaver <sweaver <@t> tvmdl.tamu.edu>wrote:

> I am in a veterinary diagnostic lab.  In the past we have had very few
> requests for IHC and have always sent slides out to another lab to perform
> IHC as needed.  It is time for us to start doing our own and join the modern
> age.  We have several certified technicians, but none have experience with
> IHC and we typically have a relatively high turnover rate.  Therefore, I am
> hoping to be able to buy an automated stainer.  In the past most people on
> the list seemed to be very happy with the Dako autostainer, but this past
> week has brought so many bad remarks about Dako's service that I am
> reconsidering.  We probably will not need a high capacity autostainer, but I
> would like walk-away capability with an easy to use system.  It will need to
> accept other companies reagents, since veterinary infectious disease
> antibodies aren't often sold by the major companies.  Also, cost is an issue
> and I would like to be able to bargain shop for reagents through other
> companies.  Does anyone have any recommendations, or warnings as to what to
> avoid?
>
> In a related issue, where do other animal tissue people get their
> antibodies for infectious diseases, e.g. Parvovirus, canine distemper, or
> FIP?
>
> Thanks for the advice!
>
>
>
>
> Stephanie Weaver
> Texas Veterinary Medical Diagnostic Lab
>
>
>
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-- 
Julie Trejo, HT(ASCP)cm

Saint Louis University
Department of Dermatology
314-256-3413