[Histonet] DI water

[LINDA MARGRAF]

Here's a message from Jennifer....(please respond to the list or her directly.... thanks)
 
Hi everyone,
   We are getting ready to start running immunos at our lab and we need to install a DI water system.  After talking to a friend who recently put in a system at his lab, I realize that there is a lot more to it than just simply putting a hole in the wall and tapping in some DI water.  Anways, we are going to start running them by hand but, eventually want to be able to use an automatic stainer.  Since the buffers require the use of DI water and it is recommended to it in the pressure cooker or decloaker for heat retrieval, we definitely need to put in a system.  We will most likely be starting out running just a handful of stains but, would like to be able to increase that number eventually. Can anyone tell me what type of water you use (ultrapure, Cap type 2 etc), how much water (gpm), how much space in your lab it requires, do you need a certain pressure, and any other helpful tips?  
I appreciate it.  Thank you.
 
Jen Campbell  
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