[Histonet] Factor XIIIa in epidermal cell nuclei

[Sate Hamza]

Greetings everyone ..

I think this may have been mentioned on this list once before in 2005
(judging from the Archives), but I would appreciate your input. Do other
people use clone AC-1A1 of Factor XIIIa? .. That's the antibody we use here
and I am not particularly comfortable with this antibody staining the nuclei
of epidermal cells. Was anybody able to fix this with that clone, or do we
just have to look for another source? .. We went down to a 1:800 dilution
and the nuclear staining is still there. At 1:1000, the staining is very
weak both in dermal dendritic cells as well as in the epidermis ..

Thank you

Sate

-- 
Sate Hamza, MD, FRCPC
Dermatopathologist
Winnipeg, Canada