[Histonet] Bad sections

[Lee Wenk]

Did you change paraffins lately? Softer paraffins, ie., lower melting point, 
have more compressibility.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

----- Original Message ----- 
From: "Va Paula Sicurello" <vapatpxs <@t> yahoo.com>
To: "HistoNet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, March 11, 2009 11:29 AM
Subject: [Histonet] Bad sections


>
> Hi Histo netters,
>
> I am am my wit's end.  I know how to section but my sections are 
> compressing like crazy.  I've altered my processing protocol thinking that 
> I was overprocessing my samples.
>
> I've tried different knife angles, different brands of razor blade knives.
>
> It's either me or the microtome.
>
> Info- I process animal tissues with between 30-60 minute steps.  Vacuum 
> paraffins for 30 minutes.  Section with Reichert-Jung 2030 set at 4-ish 
> degrees (a little below the middle line on the knife holder).
> Soak blocks on Downey ice blocks, water bath at 44 degrees.
>
> Suggestions?
>
> Waa,
>
> Paula
>
> Paula Sicurello
> VA Medical Center San Diego
> Veterans Medical Research Foundation (VMRF)
> Core Research Imaging Center
> 3350 La Jolla Village Dr., MC151
> San Diego, CA 92161
> 858-552-8585 x2397
>
>
>
>
>
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