[Histonet] Bad sections

[Merced Leiker]

What animal tissues are you using? If rodent, may need less time on the 
steps - like 10-20 min.

--On Wednesday, March 11, 2009 11:29 AM -0700 Va Paula Sicurello 
<vapatpxs <@t> yahoo.com> wrote:

>
> Hi Histo netters,
>
> I am am my wit's end.  I know how to section but my sections are
> compressing like crazy.  I've altered my processing protocol thinking
> that I was overprocessing my samples.
>
> I've tried different knife angles, different brands of razor blade knives.
>
> It's either me or the microtome.
>
> Info- I process animal tissues with between 30-60 minute steps.  Vacuum
> paraffins for 30 minutes.  Section with Reichert-Jung 2030 set at 4-ish
> degrees (a little below the middle line on the knife holder). Soak blocks
> on Downey ice blocks, water bath at 44 degrees.
>
> Suggestions?
>
> Waa,
>
> Paula
>
> Paula Sicurello
> VA Medical Center San Diego
> Veterans Medical Research Foundation (VMRF)
> Core Research Imaging Center
> 3350 La Jolla Village Dr., MC151
> San Diego, CA 92161
> 858-552-8585 x2397
>
>
>
>
>
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

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