[Histonet] new FS stain problem

[Lynette Pavelich]

I have found that the longer I delay getting the frozen section into fixative, the worse the staining/results.  

Hope this helps,
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503

ph:  810-257-9948
fax:  810-762-7082
>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 03/09/09 11:41 AM >>>
I am not completely sure but perhaps that first section taken after cutting down into the block is affected by that triming. Why don't you just discard that first section and start using the following 3 to see if they all stain corretly?
René J.

--- On Mon, 3/9/09, King, Laurie <king.laurie <@t> marshfieldclinic.org> wrote:

From: King, Laurie <king.laurie <@t> marshfieldclinic.org>
Subject: [Histonet] new FS stain problem
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, March 9, 2009, 11:05 AM

Good day all,

I am doing frozen sectioning at three different facilities in two towns, and
this problem is identical at each. 
The first section I keep, I'm in the habit of placing at the frosted end of
the slide. No matter if I take one, two, three or more, that first section is
almost non existent hematoxylin, very blurry staining. 

Thinking I was having contamination, solution level problems, I checked all
levels and made sure to have no dripping from the top of the slide, still the
same problem. Then, on a whim, I put the first section at the bottom of the
slide, and two more working my way toward the frosted end. The first section,
placed at the bottom of the slide now, still had  the poor staining. Any ideas?

I use OCT
fix in half and half formalin/95% ETOH
DW
Harris
tap
bluing
tap
eosin
95% X2
100% X2
xylene X2

Let's just say I am a "seasoned" tech, been doing this for almost
30 years, so I'm not a little embarrassed by my inability to figure this one
out.....

Laurie King
Marshfield Clinic
Eau Claire/Rice Lake Centers
Eau Claire WI





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