[Histonet] Masson's trichrome stain

[Walters, Katherine S]

I am having a problem with Biebrich Scarlet.  I am attempting to do a
Masson's stain on some mouse heart tissue.  Instead of the strikingly
bright red of the muscle tissue I am getting a purple color instead.
Everything else looks fine.  The hearts are fixed in Penfix and are
embedded in paraffin. We make the solution up fresh for each run.  I am
using Sigma's Ponceau BS (CI 26905).  Any ideas?

Katherine Walters
Histology Director
Central Microscopy Research Facilities
85 Eckstein Medical Research Building
University of Iowa
Iowa City, Iowa 52242-1101

katherine-walters <@t> uiowa.edu
www.uiowa.edu/~cemrf







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