Re: RE: [Histonet] CD31 on rat brain

[TF]

thx.
I did notice another anti-rat CD31 from Abcam (they have 41 types of anti-CD31). only mentioned works on Frozen section.

How's BD bioscience CD31 work on IHC-Frozen and paraffin sections (animals were fixed with PFA)?


2009-03-07 



TF 



发件人： Chiriboga, Luis 
发送时间： 2009-03-07  00:16:34 
收件人： tifei <@t> foxmail.com; Colleen Forster 
抄送： histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 
主题： RE: [Histonet] CD31 on rat brain 
 
For mouse (specifically) have used CD31 from BD catalog number 550274. Its a rat ant-mouse (Clone MEC13.3). 
can supply more info if need.
The problem I have found is finding good control material for this marker. especially since my only source tends to be the investigators that provide me the samples to begin with.....
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of TF
Sent: Friday, March 06, 2009 10:23 AM
To: Colleen Forster
Cc: histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
Subject: [Histonet] CD31 on rat brain
Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections?
It would be great if this also works on mice.
2009-03-06 
TF 
发件人： Colleen Forster 
发送时间： 2009-03-06  00:57:29 
收件人： tifei 
抄送： iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 
主题： Re: [Histonet] staining brain vessels 

You have to have a specific CD31 to work on mouse brain. Very few of 
them do.
Colleen Forster
TF wrote:
> hi, CD31 works great
> in my section alpha-SMA also works
>
> another way is to perfuse the brain with BSA-rhodamine.
> you will get the fluorescence without the need of staining.
>
>
> 2009-03-05 
>
>
>
> TF 
>
>
>
> 发件人： iskaliora 
> 发送时间： 2009-03-05  18:49:11 
> 收件人： histonet 
> 抄送： ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 
> 主题： [Histonet] staining brain vessels 
>  
> I was wondering if anybody might have an idea with the following  
> problem we are experiencing:  we want to stain for blood vessels in  
> sections of mouse brain.  Our experimental tissues have been fixed  
> overnight in 4% paraformaldehyde and have been sitting in PBS since.   
> We have tried staining with antibodies against desmin, SMA, and  
> collagen but we get NO specific signal.  We recently tried a non-fixed  
> mouse brain and got desmin to work immediately.  The problem is that  
> we need to use the fixed brains because they are our experimental  
> model and it would take too long (2 years to be exact) to generate the  
> same samples.  If anybody has come across such a problem before, or  
> has a specific protocol for vessels that works on PFA fixed brain, we  
> would appreciate the suggestions!
> thanks in advance!
> Irini
> -----------------------------------------------------
> Irini Skaliora, PhD
> Investigator C᾽ (Assistant Professor)
> Developmental Biology Division
> Biomedical Research Foundation of the Academy of Athens (BRFAA)
> Soranou Efessiou 4
> Athens 11527
> tel. +30-210-6597203 (office)
> tel. +30-210-6597482 (lab)
> fax. +30-210-6597545
> email: iskaliora <@t> bioacademy.gr
> _______________________________________________
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