[Histonet] Re: GFP antibody
TJJ <@t> stowers.org
Fri Mar 6 09:44:17 CST 2009
We have had success staining murine paraffin sections using Rockland goat anti-GFP antibody.
You'll need to optimize it to your laboratory conditions trying with either antigen retrieval (citrate buffer pH 6.0) or Proteinase K. Make sure you have tissue with known positive GFP expression and a wild type (negative) animal for controls.
As for whether processing destroys the GFP in the sample, it will render the protein non-fluorescent. Fixation alone can do that. But the protein should still be there, even if it is not glowing.
Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110
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