AW: [Histonet] staining for elastic fiber of artery in rat lung

[Gudrun Lang]

Do you know or mean the Verhoeff Elastin stain?

Verhoeff staining solution:
25 ml 10% Haematoxylin in abs. ethanol
25 ml abs. ethanol
25 ml 10% FeCl3 (CAS 7705080)
Mix well and ad
25 ml Verhoeff iodinsolution (=2 g Iodine + 4 g KI + 100 ml water)

1. deparaffinize and rehydrate slides
2. Verhoeff staining solution 20 min
3. lukewarme tapwater 5 min
4. differentiate in 2% FeCl3 1-2 min
5. rinse in Aqua dest.
6. rinse in 5% Sodiumthiosulfate 1 min
7. rinse in running tapwater
8. counterstain as you like
9. dehydrate, clear and coverslip

Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Amy Lee
Gesendet: Mittwoch, 04. März 2009 19:22
An: histonet
Betreff: [Histonet] staining for elastic fiber of artery in rat lung 

Hello histonetters,
 
Would you teach me what is best stain for elastic fiber in rat lung except
weigert's stain?
 
I want to look at arteries.
 
Thanks in advance,
Weihua


      
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