[Histonet] AXOn staining

[TF]

Re: 
Hi Nancy, do Tuj1 (beta 3 tubilin) stain nicely in adult rat brain? I think Tuj1 is restricted to immature neurons inside the brain ! though it stains well with retinal ganglion cell....

I am also seeking for a nice marker for axon ...both normal and regenerating...GAP-43 is one choice for regenerating axons...but a bit focused on the growth cone, and the staining is not strong on transporting proteins - due to the tiny amount? I am not sure how NF-200 or NF-96/160 work....

ANyone can suggest better markers for axon IHC staining? both normal and regenerating.

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Axons stain nicely with beta 3 tubuline (TUJ). We've used the following
antibodies
Monoclonal anti-beta-tubulin isotype III from Sigma  1/1000  DAKO envision
(révélation PAL or HRP) (ref : T8660)
or
Polyclonal  (rabbit)  antibody against neuronal class III beta-tubulin from
covance  research  commercialise  par  BABCO  Berkeley  antibody company au
1/1000 DAKO envision (révélation PAL or HRP) (ref : PRB-435P)

We  used  paraffin  slices  of  PFA  fixed rat embryos and adult rat brain,
microwaving in citrate buffer. These antibodies also work nicely with IF

Good luck!
Nancy Walker
Molecular Biology Scientist

Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE

nancy.walker <@t> sanofi-synthelabo.com
tel : (33)561004179  fax :(33)561004001



2009-03-05 



TF