[Histonet] vector blue- xylene soluble- any alternatives

[Patsy Ruegg]

If I am not mistaken vectamount is an aqueous mountant, dpx is
xylene/toluene based and requires going thru alcohols and xylene to mount.
One thing I do and you can try it, is airdry the slides and the use
permanent mounting media without going thru alcohols and xylene, keep in
mind there is a little solvent in the media so it may have an effect.  I do
this with Fast Red substrate chromogen for AP have not tried it on vector
blue.  The other option is to use an aqueous mountant and seal the coverslip
edges with clear nail polish.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of anjan kumar
Sent: Wednesday, March 04, 2009 12:05 PM
To: histonet-requests <@t> lists.utsouthwestern.edu; triple immunohistochem
Subject: [Histonet] vector blue- xylene soluble- any alternatives


hello everyone,

                    i have a problem which i dont know how to tackle, the
problem is that i have purchased vector blue as a chromogen and it is xylene
soluble can anyone suggest me how to avoid this.... as funds are low i cant
purchase xylene substitutes. 

 

second question been, ...sorry this has been a addition ......

what is the difference between the DPX mountant and other permanent mounting
media like vectamount. kindly answer these questions...

 

regards,

Anjan Kumar

Junior research scientist

Veterinary Pathology

Madras veterinary college.

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