[Histonet] problem eyes

[Rene J Buesa]

Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it..
René J.

--- On Wed, 3/4/09, Va Paula Sicurello <vapatpxs <@t> yahoo.com> wrote:

From: Va Paula Sicurello <vapatpxs <@t> yahoo.com>
Subject: Re: [Histonet] problem eyes
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>, "MargaretPerry" <Margaret.Perry <@t> sdstate.edu>, rjbuesa <@t> yahoo.com
Date: Wednesday, March 4, 2009, 4:23 PM

Really?  Liquid laundry soap?  Does this help with all wrinkles in large
specimens?


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Wed, 3/4/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] problem eyes
> To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Perry, Margaret"
<Margaret.Perry <@t> sdstate.edu>
> Date: Wednesday, March 4, 2009, 9:14 PM
> Add a few drops (4-5) of liquid
> detergent, but not dishwasher detergent (because it will
> dissolve the paraffin).
> rené J.
> 
> --- On Wed, 3/4/09, Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
> wrote:
> 
> From: Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
> Subject: [Histonet] problem eyes
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, March 4, 2009, 4:04 PM
> 
> Please help!  We have been trying to cut whole eyes
> from a pig.  They need to be
> nice enough for a publication.  We are having the
> devil of a time because no
> matter what we do there are wrinkles.  If we turn the
> waterbath up to stretch
> things out the retina detaches.  Do any of you have
> suggestions?  We uses
> Surgipath  EM400 for embedding paraffin and and their
> infiltration medium.  Is
> this to soft? The lowest we can turn our waterbath is 38
> degrees C.
> 
> Margaret Perry HT (ASCP)
> IHC Lab Manager Veterinary Science
> Animal Disease Research and Diagnostic Lab
> South Dakota State University
> Box 2175 North Campus Drive
> Brookings SD 57007
> 
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