[Histonet] Storage of muscles

[Hofecker, Jennifer L]

I agree with Tony that a -70 freezer is ideal. We wrap our samples in aluminum foil and then place in a plastic container. If you only have access to a -20 freezer, it's helpful to place cold packs in the freezer, near where you will store the muscle samples. When a -20 freezer cycles, the temperature can fluctuate enough to create artifact. We've found that if we keep several disposable cold packs in a -20 that we can keep the temperature more consistent. 
Good Luck!

Jennifer L. Hofecker HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
Nashville, TN 
ph 615.343.0083
fax 615.343.7089

-----Original Message-----
From: Tony Henwood [mailto:AnthonyH <@t> chw.edu.au] 
Sent: Tuesday, March 03, 2009 3:59 PM
To: rennie1108 <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Storage of muscles

Minus 70 degrees
A normal minus 20 freezer tends to dessicate the tissue

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
Sent: Wednesday, 4 March 2009 7:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Storage of muscles


Hello,
We are having problems with artifact after our muscles have been stored for long periods of time. Can anyone tell me how they store their muscle specimens for long-term?
 
Thank you,
Adrienne Anderson
Duke University Health System
rennie1108 <@t> yahoo.com
 


      
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