[Histonet] Cresyl violet problems

[Walters, Katherine S]

To the "brainy" histo-people,

I have a student doing a cresyl violet stain on some frozen (40um) brain
sections.  He has been doing this routinely for a couple of months, but
his last run looks very odd.  There appears to be an area on each
section where there is no staining.  At first glance I thought it was a
fixation problem, but the unstained portion of the tissue is in a
different area as you move from slice to slice (these are serial
sections),   He stains in a Copeland jar and assures me that his stain
is well homogenized.  Could this be some kind of moisture problem?

Thanks for your thoughts.


Katherine Walters
Histology Director
Central Microscopy Research Facilities
85 Eckstein Medical Research Building
University of Iowa
Iowa City, Iowa 52242-1101

katherine-walters <@t> uiowa.edu
www.uiowa.edu/~cemrf







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