[Histonet] Section thickness

[Houston, Ronald]

3 microns, but 2 micron for lymphoid tissues and renal biopsies

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan
Shivers
Sent: Tuesday, March 03, 2009 10:17 AM
To: Vanessa J. Phelan; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Section thickness

4 um here.

Jan Shivers
UMN VDL

----- Original Message ----- 
From: "Vanessa J. Phelan" <vjp2105 <@t> columbia.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, March 03, 2009 8:14 AM
Subject: [Histonet] Section thickness


> Hi Guys,
>
> Just wondering what thickness you cut sections at? I was always used
to
> cutting at 2-3 microns in my last lab, however in my new place they
are
> cutting at 6 microns (for both H & Es and IHC), which seems to me as 
> really
> quite thick! What would be the average cutting thickness?
>
> Thanks a mill,
>
> Vanessa
>
>
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