[Histonet] RE: Signs of good perfusion

[Merced M Leiker]

but he's not using fixative.

--On Tuesday, June 30, 2009 10:06 AM +0100 "Edwards, R.E." 
<ree3 <@t> leicester.ac.uk> wrote:

> A stiff  tail!.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thach,
> Dzung (NIH/NIAID) [E] Sent: 29 June 2009 16:31
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Signs of good perfusion
>
> Hi Everyone!!
>
>    I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
> nicking the upper right atrium of heart to collect the gushed out blood
> and then perfusing through ventricle using a 21G butterfly needle and
> peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes
> out of mouth.  Occasionally, I see the liver fade to light pink.  Most of
> the time the paws become white.  But the brain and spinal cord (my
> tissues of interest) are always white, and seem to have been perfused.  I
> was wondering how to improve this to get more consistent good perfusions,
> and what signs should I look for to indicate good perfusion?
>
> Thanks much,
>
> Dzung
> NIAID
>
>
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY  14214
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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