[Histonet] Sevier-Munger question for special stain experts
Rene J Buesa
rjbuesa <@t> yahoo.com
Wed Jun 24 11:43:17 CDT 2009
I always tried to handle slides to be stained with silver individually, but if you decide to go for the whole batch, yes you can multiply the components, any way the "end point" of the solution will tell you when it is correct.
--- On Wed, 6/24/09, Andrea Grantham <algranth <@t> email.arizona.edu> wrote:
From: Andrea Grantham <algranth <@t> email.arizona.edu>
Subject: [Histonet] Sevier-Munger question for special stain experts
To: "HISTONET" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, June 24, 2009, 12:36 PM
If there is anyone out there who does silver stains in large batches I need your input.
I haven't done a Sevier-Munger stain in probably 20 years and when I did this kind of stain I usually had 2-3 slides to stain. So now I have a request to do this stain and I have multiple racks of slides.
What is the best way to handle the ammoniacal silver step - each slide individually or plunk a whole rack of slides into a staining dish? And if I do the latter, can I multiply the amounts by 5 to make 250 ml. of the ammoniacal silver solution which is the amount needed to fill the staining dish?
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
Tucson, AZ 85724
algranth <@t> email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097
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