[Histonet] sections fading
laurie.reilly <@t> jcu.edu.au
Sun Jun 21 18:19:49 CDT 2009
Talking of sections fading --
I am having trouble with my Grams fading.
We do Gram Twort on paraffin sections and they stain beautifully, but after
3 or 4 days the Gram positive bugs are no longer Gram positive.
Can anyone help?
Thanks and regards, Laurie.
Mr. Laurie REILLY
School of Veterinary and Biomedical Sciences
James Cook University
Townsville Qld. 4811
Phone 07 4781 4468
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl
Sent: Monday, 22 June 2009 2:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] re:xylene and frozen sections
Imho, if one does not use Xylene that is low in sulphur, there is a good
chance that your eosin staining will fade, whether on frozen or Pwax
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