[Histonet] sections fading

[Laurie Reilly]

Talking of sections fading --
I am having trouble with my Grams fading.
We do Gram Twort on paraffin sections and they stain beautifully, but after
3 or 4 days the Gram positive bugs are no longer Gram positive.

Can anyone help?

Thanks and regards,     Laurie.

Mr. Laurie REILLY
Histopathology
School of  Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl
Sent: Monday, 22 June 2009 2:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] re:xylene and frozen sections

Imho, if one does not use Xylene that is low in sulphur, there is a good
chance that your eosin staining will fade, whether on frozen or Pwax
sections...


carl
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