[Histonet] RE: Diff Quik II
Judy Keller
jkeller <@t> schospital.com
Fri Jun 19 15:05:29 CDT 2009
We buy Diff Quik II by the gallon directly from Dade.
You don't need the kit.
Judy Keller, HTL
South County Hospital
Wakefield, RI
________________________________
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Sent: Fri 6/19/2009 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 67, Issue 19
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Today's Topics:
1. Synaptophysin (Benny Babinsack)
2. biocare bcl 6 (Orr, Rebecca)
3. Cytokeratin 18 M30 apoptosis staining (Popp, Laurie A.)
4. RNA In Situ Hybridizaton (Bell, Pat)
5. FW: Cytokeratin 18 M30 apoptosis staining (Popp, Laurie A.)
6. Glypican-3 on BondMax (Steven Wilkes)
7. Glypican-3 on BondMax (Steven Wilkes)
8. Glypican-3 on BondMax (Steven Wilkes)
9. xylene and frozen sections (Lynn Dike)
10. Temp work (Loralei Dewe)
11. Diff Quik II (Jessica Piche)
12. reticulin stain (Derek Papalegis)
13. reticulin stain (Rene J Buesa)
14. RE: Diff Quik II (Jodie Robertson)
15. Re: anti Brdu (Reynolds,Donna M)
16. Ventana Reagent Cost?? (Tiana Fountain)
17. Good Used Equipment for Sale (Denise Van Eaton)
18. Mouse necropsy question (Thach, Dzung (NIH/NIAID) [E])
19. Re: Ventana Reagent Cost?? (Rene J Buesa)
20. RE: Ventana Reagent Cost?? (Burton, Lynn)
----------------------------------------------------------------------
Message: 1
Date: Mon, 15 Jun 2009 11:54:04 -0400
From: Benny Babinsack <BBabinsack <@t> genesishcs.org>
Subject: [Histonet] Synaptophysin
To: histonet <@t> lists.utsouthwestern.edu
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OF8F32BEEA.EB47166F-ON852575D6.00575917-852575D6.00575919 <@t> genesishcs.org>
Content-Type: text/plain; charset="ISO-8859-1"
We use a IVD from Cellmarque and use pa ncreas for the control
Benny Ba Lab Services Senior Tech,&n (740)454-5587
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------------------------------
Message: 2
Date: Tue, 16 Jun 2009 12:29:47 -0500
From: "Orr, Rebecca" <ROrr <@t> northshore.org>
Subject: [Histonet] biocare bcl 6
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Cc: "'JPeters <@t> bostwicklaboratories.com'"
<JPeters <@t> bostwicklaboratories.com>
Message-ID:
<B44CA3426A2C084F9BA90B93D528159BE4E2CB7A <@t> EXCH34.enhnet.org>
Content-Type: text/plain; charset="us-ascii"
Message: 3
Date: Mon, 15 Jun 2009 15:34:33 -0400
From: "Justin Peters" <JPeters <@t> bostwicklaboratories.com>
Subject: [Histonet] BCL-6
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<24D22DE9E488AA43BF92A4389F2DDB1F05DFAF54 <@t> mail1.BOSTWICK.COM>
Content-Type: text/plain; charset="us-ascii"
I am having trouble getting bcl-6 antibody to work (Biocare CM223).
Does anyone have a good protocol for me to try?
Hi Justin,
I run BCl-6 from concentrate 1:50 with Biocare's RED diluent with a Decloaker using RED diluent.
Most antibodies perform well with the "green" diluent; this particular antibody is best using the "RED".
I also use Mach4 detection... if problems persist, contact me offline, I may be able to help with some other suggestions.
Hope this helps.
Becky Orr CLA,HT(ASCP)QIHC
Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem
847-570-2771
*********************
------------------------------
Message: 3
Date: Tue, 16 Jun 2009 15:59:46 -0500
From: "Popp, Laurie A." <Popp.Laurie <@t> mayo.edu>
Subject: [Histonet] Cytokeratin 18 M30 apoptosis staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1488342ADB74EC4E969C48BDCDE0BD98FB7735 <@t> msgebe23.mfad.mfroot.org>
Content-Type: text/plain; charset="US-ASCII"
Has anyone worked with this particular antibody? Can you give me any
advise as far as what worked? I have an investigator that wants to use
this antibody in diseased liver tissue and nothing that I am trying is
working.
Thanks!
Laurie
Laurie Popp, BA HT ASCP cm
TACMA Shared Services
Mayo Clinic-Rochester, MN
------------------------------
Message: 4
Date: Tue, 16 Jun 2009 15:05:41 -0600
From: "Bell, Pat" <Pat.Bell <@t> ucdenver.edu>
Subject: [Histonet] RNA In Situ Hybridizaton
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<64DB27005E2FD3439E88502D7A5C9121877CB00392 <@t> CORTEZ.ucdenver.pvt>
Content-Type: text/plain; charset="us-ascii"
My supervisor wants me to learn In Situ Hybridization using RNA probes in formalin fixed, paraffin embedded tissues. I have a Dako Autostainer, but I know that some of the procedure would be manual.
I would appreciate any protocols that would help me.
Thank you for your help.
Pat
Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
Aurora, Co. 80045
303-724-6077
pat.bell <@t> ucdenver.edu<mailto:pat.bell <@t> ucdenver.edu>
------------------------------
Message: 5
Date: Wed, 17 Jun 2009 12:08:08 -0500
From: "Popp, Laurie A." <Popp.Laurie <@t> mayo.edu>
Subject: [Histonet] FW: Cytokeratin 18 M30 apoptosis staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1488342ADB74EC4E969C48BDCDE0BD98FB7739 <@t> msgebe23.mfad.mfroot.org>
Content-Type: text/plain; charset="US-ASCII"
> Has anyone worked with this particular antibody? Can you give me any
> advise as far as what worked? I have an investigator that wants to
> use this antibody in diseased liver tissue and nothing that I am
> trying is working.
>
> Thanks!
> Laurie
>
> Laurie Popp, BA HT ASCP cm
> TACMA Shared Services
> Mayo Clinic-Rochester, MN
>
>
------------------------------
Message: 6
Date: Thu, 18 Jun 2009 15:38:22 -0400
From: Steven Wilkes <srwilkes <@t> gmail.com>
Subject: [Histonet] Glypican-3 on BondMax
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<8e5827cf0906181238x6ee4b038hc98ea547ee2939a <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Histonetters!
Has anyone been using Glypican-3 on the Leica BondMax platform? If so,
please msg me, as I have some questions. I am having a tough time finding
the right conditions... dilution, pretreatment, incubation times etc..
thanks
Steven
srwilkes <@t> gmail.com
------------------------------
Message: 7
Date: Thu, 18 Jun 2009 15:42:12 -0400
From: Steven Wilkes <srwilkes <@t> gmail.com>
Subject: [Histonet] Glypican-3 on BondMax
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<8e5827cf0906181242nf88a092o4a3868a7b4ba3dd7 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Histonetters!
Has anyone been using Glypican-3 on the Leica BondMax platform? If so,
please msg me, as I have some questions. I am having a tough time finding
the right conditions... dilution, pretreatment, incubation times etc..
thanks
Steven
srwilkes <@t> gmail.com
------------------------------
Message: 8
Date: Thu, 18 Jun 2009 15:39:27 -0400
From: Steven Wilkes <srwilkes <@t> hotmail.com>
Subject: [Histonet] Glypican-3 on BondMax
To: histology jobs <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY101-W5061A0061CCA94443AB33AC13D0 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
Hi Histonetters!
Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks
Steven
srwilkes <@t> gmail.com
_________________________________________________________________
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------------------------------
Message: 9
Date: Thu, 18 Jun 2009 19:47:14 -0500
From: Lynn Dike <lynnd01 <@t> hotmail.com>
Subject: [Histonet] xylene and frozen sections
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU129-W6D6EF256F9F660349B97ABF3A0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Lynn
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Message: 10
Date: Thu, 18 Jun 2009 18:30:09 -0700
From: Loralei Dewe <lldewe <@t> gmail.com>
Subject: [Histonet] Temp work
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<7173d3c00906181830o27dfc5d3sbd8cc8d5050066e5 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi everyone,
I am looking for temp work. If you have availability on a travel basis let
me know. I have 10+ years experience with histology and IHC in a research
venue and am published!
Cheers,
Loralei
------------------------------
Message: 11
Date: Fri, 19 Jun 2009 05:51:58 -0700 (PDT)
From: Jessica Piche <jessgrocki <@t> yahoo.com>
Subject: [Histonet] Diff Quik II
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <150374.59014.qm <@t> web82008.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Good Morning!
I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks!
Happy Friday!
Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital
------------------------------
Message: 12
Date: Fri, 19 Jun 2009 09:11:05 -0400
From: Derek Papalegis <derek.papalegis <@t> tufts.edu>
Subject: [Histonet] reticulin stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4A3B8E69.3090403 <@t> tufts.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
I am having some difficulty getting the proper results from my reticulin
stain. I have been following and tweaking the procedure outlined in
Carson's book without any success. Could someone please forward me their
procedure that has all the kinks worked out?
Thanks,
Derek
--
Derek Papalegis HT (ASCP)
Senior Histology Technologist
Division of Laboratory Animal Medicine
Tufts University
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354
------------------------------
Message: 13
Date: Fri, 19 Jun 2009 06:26:34 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: [Histonet] reticulin stain
To: histonet <@t> lists.utsouthwestern.edu, Derek Papalegis
<derek.papalegis <@t> tufts.edu>
Message-ID: <431812.3131.qm <@t> web65714.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Derek:
I am sending it to you under separate cover.
René J.
--- On Fri, 6/19/09, Derek Papalegis <derek.papalegis <@t> tufts.edu> wrote:
From: Derek Papalegis <derek.papalegis <@t> tufts.edu>
Subject: [Histonet] reticulin stain
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, June 19, 2009, 9:11 AM
I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out?
Thanks,
Derek
-- Derek Papalegis HT (ASCP)
Senior Histology Technologist
Division of Laboratory Animal Medicine
Tufts University 136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354
_______________________________________________
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------------------------------
Message: 14
Date: Fri, 19 Jun 2009 06:49:52 -0700
From: "Jodie Robertson" <jrobertson <@t> pathologysciences.com>
Subject: RE: [Histonet] Diff Quik II
To: "Jessica Piche" <jessgrocki <@t> yahoo.com>, "histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<518CD6920AA7154193CBE5977CD880733A8A7C <@t> psmgsrv2.PSMG.local>
Content-Type: text/plain; charset="iso-8859-1"
I purchase Diff Quik Solution II in a 2.5L bottle from Cardinal Health Catalog # B4132-12A. It's not something that they have in stock and it usually ships from Dade Behring Inc. which is who makes it. It usually takes me a couple of weeks to get it, but I have no problem receiving it.
Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jessica Piche
Sent: Friday, June 19, 2009 5:52 AM
To: histonet
Subject: [Histonet] Diff Quik II
Good Morning!
I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks!
Happy Friday!
Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Fri, 19 Jun 2009 09:28:09 -0500
From: "Reynolds,Donna M" <dreynold <@t> mdanderson.org>
Subject: [Histonet] Re: anti Brdu
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<785BBF0C5F49CE41BA74460A43A08F0214492216F0 <@t> DCPWVMBXC0VS3.mdanderson.edu>
Content-Type: text/plain; charset="us-ascii"
We have used mouse anti-Brdu from Becton Dickinson for years. It is still available from BD BioSciences Cat. 347580. I know it is made in mouse but we used an IgG1 secondary and always had good results. There may be some cytoplasmic label, probably background, but you are interested in the nuclear staining and it is very distinct. We have used it on both frozen and paraffin processed tissues.
Donna Reynolds, HT (ASCP)
Dept. Cancer Biology
U.T. M.D. Anderson Cancer Center, Houston Tx
Message: 3
Date: Wed, 17 Jun 2009 14:26:09 -0400
From: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>
Subject: [Histonet] source for rat anti mouse brdu?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E5B1C3D1305EFD4C9CA7F63094135F5E0226AC30 <@t> MD1EV002.medimmune.com>
Content-Type: text/plain; charset="us-ascii"
I have not done a brdu on mouse in five years. Used Harlan but they are
not selling that anymore. I used a procedure using rat anti mouse brdu
primary, rabbiot anti rat mouse adsorbed secondary, then ABC/DAB. Anyone
have any idea on a good source? I used biocompare and found stuff but
would like to hear comments. Also anyone use conjugated brdu?
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr,
OMW, Area 4, Lab 2438
301.398.4745(vm)
301.398.6360(lab)
301.398.9745(fax)
*****************
------------------------------
Message: 16
Date: Fri, 19 Jun 2009 10:42:09 -0500
From: "Tiana Fountain" <tfountain <@t> exchange.hsc.mb.ca>
Subject: [Histonet] Ventana Reagent Cost??
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BF2F7B1BBD30C5478EF41CF80164D99C7A7D16 <@t> hscxmsmx0010.ad.wrha.mb.ca>
Content-Type: text/plain; charset="us-ascii"
Hello everyone,
I am looking for a cost of some of the Ventana reagents can anyone help?
Specifically:
LCS:
CC1:
CC2:
EZ Prep:
Reaction Buffer:
I am not currently a customer so I didn't want to phone Ventana directly
if I don't have to. Thanks
Tiana
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Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original.
------------------------------
Message: 17
Date: Fri, 19 Jun 2009 10:52:15 -0500
From: Denise Van Eaton <dsv22 <@t> hotmail.com>
Subject: [Histonet] Good Used Equipment for Sale
To: histonetmailinglist <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT105-W31D98ABB65544F42A33748AE3A0 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
Good Morning HistoNetters- Good Used equipment for sale!
We have a Leica TP1050 bench model that is currently being used on a daily basis. The 300 cassette capacity retort, will process with or without heat, with or without pressure or vacuum. It has the reagent management system to assist with "rotation" of reagents as well as the xylene extraction cycle for the paraffin. We purchased it "used" about 2 years ago and it has worked very well for us. We just need the space more than the additional processor.
We also have a 4+ year old Dako Autostainer. Each run will hold 44 slides. It is an open system, and so will allow you to use whatever reagents you choose. This unit has worked flawlessly for us, but we are discontinuing our on-site IHC program.
Both of these units have been well maintained, are sold "as is" and will need to be picked up by the purchaser. We have no shipping crates and/or the muscles to pack up either machine. We are located just east of the Kansas City, Missouri area in Blue Springs. Anyone interested, please contact Denise Van Eaton @ dvaneaton <@t> littonlab.com
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Message: 18
Date: Fri, 19 Jun 2009 11:56:27 -0400
From: "Thach, Dzung (NIH/NIAID) [E]" <thachdc <@t> niaid.nih.gov>
Subject: [Histonet] Mouse necropsy question
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C6612D6B.D07%thachdc <@t> niaid.nih.gov>
Content-Type: text/plain; charset="iso-8859-1"
Hi Everyone!!
I am collecting spleen, brain, and spinal cord from virus infected mouse.
I will perfuse mouse with PBS. Do you think I need to use a new pair of
scissors and forceps for each tissue in each mouse and between each mouse?
Thanks,
Dz
------------------------------
Message: 19
Date: Fri, 19 Jun 2009 08:57:20 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Ventana Reagent Cost??
To: histonet <@t> lists.utsouthwestern.edu, Tiana Fountain
<tfountain <@t> exchange.hsc.mb.ca>
Message-ID: <737537.89352.qm <@t> web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
`If you don't use their auto stainer probably you will not be able to use their antibodies that, as far as I know, are "optimized" to be used in their instrument in what they call "liquid cover slips" for room and high temperature.
René J.
--- On Fri, 6/19/09, Tiana Fountain <tfountain <@t> exchange.hsc.mb.ca> wrote:
From: Tiana Fountain <tfountain <@t> exchange.hsc.mb.ca>
Subject: [Histonet] Ventana Reagent Cost??
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, June 19, 2009, 11:42 AM
Hello everyone,
I am looking for a cost of some of the Ventana reagents can anyone help?
Specifically:
LCS:
CC1:
CC2:
EZ Prep:
Reaction Buffer:
I am not currently a customer so I didn't want to phone Ventana directly
if I don't have to. Thanks
Tiana
-----Inline Attachment Follows-----
This email and/or any documents in this transmission is intended for the
addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original.
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------------------------------
Message: 20
Date: Fri, 19 Jun 2009 11:46:37 -0500
From: "Burton, Lynn" <Lynn.Burton <@t> Illinois.gov>
Subject: RE: [Histonet] Ventana Reagent Cost??
To: "Tiana Fountain" <tfountain <@t> exchange.hsc.mb.ca>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B34ED7468185B44AB6CECF566B1960D001D879BF <@t> IL084EX110.illinois.gov>
Content-Type: text/plain; charset="iso-8859-1"
The prices I have are on the list you sent. I couldn't find the CC2 price quickly.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Tiana Fountain
Sent: Fri 6/19/2009 10:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Reagent Cost??
Hello everyone,
I am looking for a cost of some of the Ventana reagents can anyone help?
Specifically:
LCS:$98.40
CC1:$565.80
CC2:
EZ Prep:$37.80
Reaction Buffer:$43.80
I am not currently a customer so I didn't want to phone Ventana directly
if I don't have to. Thanks
Tiana
------------------------------
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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