[Histonet] Electron Microscopy

[Diego]

Hi,

   I am trying to get a a pre-embedding immuno to work. I have tried 
several different things (fixatives, incubation times, +/- detergent) 
and it seems that definitely I need to have a detergent present. I used 
0.05% Tween20, but the quality of the EM is really bad (it is impossible 
to distinguish anything from them).  Does anybody have experience doing 
this? Any protocol I could try? Suggestions?

   Thanks very much in advance for any help.
   Diego.

-- 

Diego J. Rodriguez Gil
Postdoctoral Associate
Department of Neurosurgery
Yale University School of Medicine
PO Box 208082
333 Cedar St, FMB-430
New Haven CT, 06520-8082
U.S.A.
Phone: 203-785-4230