[Histonet] Electron Microscopy

Diego diego.rodriguezgil <@t> yale.edu
Fri Jun 19 13:23:36 CDT 2009


   I am trying to get a a pre-embedding immuno to work. I have tried 
several different things (fixatives, incubation times, +/- detergent) 
and it seems that definitely I need to have a detergent present. I used 
0.05% Tween20, but the quality of the EM is really bad (it is impossible 
to distinguish anything from them).  Does anybody have experience doing 
this? Any protocol I could try? Suggestions?

   Thanks very much in advance for any help.


Diego J. Rodriguez Gil
Postdoctoral Associate
Department of Neurosurgery
Yale University School of Medicine
PO Box 208082
333 Cedar St, FMB-430
New Haven CT, 06520-8082
Phone: 203-785-4230

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