[Histonet] Re: Inactivation of DAB

Lynette Pavelich lpaveli1 <@t> hurleymc.com
Wed Jun 17 09:05:13 CDT 2009

We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE
HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184.

1. Prepare the following aqeous stock solutions:
   a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter)
   b.  2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter)
2. Dilute DAB solution of necessary so that its concentration does not
exceed 0.9 mg/ml.
3. For each 10ml of DAB solution, add:
   a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter)
   b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%)
4. Allow mixture to stand for at least 10 hours.  It is now
5. Decolorize the mixture with ascorbic acid (add powder until color
disappears).  This too is an oxidation/reduction reaction.
6. Neutralize the decolorized mixture with sodium bicarbonate (test with
pH meter, pH paper or dipsticks).  Note:  we recommend using magnesium
hydroxide/oxide instead of sodium bicarbonate.
7. Discard the solution down the drain provided that local wastewater
authorities have given their approval.

Hope this helps,

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com
ph:  810-257-9948
fax:  810-762-7082
>>> "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG> 06/17/09 9:30 AM >>>
We have started doing routine immunohistochemistry on our stainer and
have a
large waste container that includes diaminobenzidine (DAB).  
We would like to inactivate it so we don't have to pay to have the waste
removed.  Can anyone help me?  What do people use to inactivate their

Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (W224) 
Massachusetts General Hospital 
55 Fruit Street 
Boston, MA 02114-2696 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-1206 (fax) 
msherwood <@t> partners.org 

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