[Histonet] Re: F4/80 mouse paraffins

Reynolds,Donna M dreynold <@t> mdanderson.org
Fri Jun 12 08:32:07 CDT 2009

We have used rat anti-mouse F4/80 from Cell Sciences, clone BM8 on mouse FFPE tissue successful. We used a 1:50 dilution and Proteniase K (Dako) for antigen retrieval.  Our secondary was BioCare Medical's 4+ biotinylated detection system.  It did take a longer than normal development time in the DAB but labeling was very nice.  You could give this a try with your antibody. Good luck.

Donna Reynolds, Chief Histology Laboratory
Cancer Biology, SRB 1.660

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