[Histonet] temp histo position at Medimmune in Gaithersburg

Tue Jun 9 14:31:03 CDT 2009

We have an immediate opening for a temporary histotech at Medimmune HQ in Gaithersburg, MD outside of DC.  No relocation.  Mainly routine paraffin histology on rodent tissues, lots of it! Processing, thru HE and Specials, IHC, frozen and necropsy experience a plus. Email me if you want further details. madaryj <@t> medimmune.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, June 09, 2009 1:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 67, Issue 9

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Today's Topics:

   1. Re: Modified Davidson's fixative use (Jackie M O'Connor)
   2. Re: Modified Davidson's fixative use (Robert Richmond)
   3. quenching auto-fluorescence (Jennifer Anderson)
   4. Re: Etching plastic for T blue staining (gayle callis)
   5. Quenching autofluorescence (gayle callis)
   6. Preservation/transport medium for tissue culture (Goodwin, Diana)
   7. Low pH HIER -- Tris-HCl buffer (emerald_lake77 <@t> yahoo.com)
   8. FW: new reference books (Lena Spencer)
   9. RE: RE: [Histonet] Storage of tissues in PFA
      beforedehydrationandembedding (Tony Henwood)
  10. RE: Preservation/transport medium for tissue culture
      (Tony Henwood)
  11. Re: MAP2 and GFAP double staining (Hobbs, Carl)
  12. Histology Manager opportunity in Connecticut  (Melissa Ribeiro)
  13. Gram Stain (Laurie Colbert)
  14. antibody recommendation (Gudrun Lang)

----------------------------------------------------------------------

Message: 1
Date: Mon, 8 Jun 2009 12:06:13 -0500
From: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Modified Davidson's fixative use
To: "Johnson, Teri" <TJJ <@t> stowers.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<OF9B14DBD4.58924504-ON862575CF.005D1E9A-862575CF.005DFB00 <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"

To not answer your question - we fix eyes and testes in Modified Davidsons 
for 48 hours then transfer to formalin to await processing. 
Since mDF is basically formalin, alcohol, and gaa - a water rinse step is 
pretty moot.

"Johnson, Teri" <TJJ <@t> stowers.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/08/2009 10:56 AM

To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Modified Davidson's fixative use

Dear colleagues,

For those who use modified Davidson's fixative for your samples, do you do 
a water rinse prior to starting them in alcohol dehydration series, or 
simply go from fixative into the alcohol?

Thanks!

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

_______________________________________________
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------------------------------

Message: 2
Date: Mon, 8 Jun 2009 13:25:34 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Modified Davidson's fixative use
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<abea52a60906081025r26ef4aaambf07f208a3a0f501 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Teri Johnson, HT(ASCP)QIHC, Managing Director Histology Facility,
Stowers Institute for Medical Research in Kansas City, Missouri asks:

>>For those who use modified Davidson's fixative for your samples, do you do a water rinse prior to starting them in alcohol dehydration series, or simply go from fixative into the alcohol?<<

When I use modified Davidson's fixative as a disclosing fixative for
lymph nodes in colon resection specimens, I'll wash the tissue briefly
to reduce the smell, since my gross desk is unventilated (long story).
The cassettes containing the dissected lymph nodes will go into
neutral buffered formalin at least briefly before being processed
through alcohols.

Bob Richmond
Samurai Pathologist
Knoxville TN

------------------------------

Message: 3
Date: Mon, 8 Jun 2009 10:28:02 -0700
From: "Jennifer Anderson" <janderson <@t> halozyme.com>
Subject: [Histonet] quenching auto-fluorescence
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<A037532CC3E3974CB0AB2420B928DACD026D05C6 <@t> HTIEXCHANGE.hti.com>
Content-Type: text/plain;	charset="US-ASCII"

Good morning.

I'm wondering how to quench auto-fluorescence in tissue from a mouse
that was treated with a FITC-labeled protein.  What do people usually
use to quench the endogenous auto-fluorescence, and will this treatment
quench the FITC signal from the labeled protein?

Thanks a lot for your help!

Jennifer M. Anderson, Scientist

Halozyme Therapeutics, Inc.

11388 Sorrento Valley Road

San Diego, CA 92121

858-704-8333

janderson <@t> halozyme.com <mailto:janderson <@t> halozyme.com> 

The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.

------------------------------

Message: 4
Date: Mon, 8 Jun 2009 12:19:43 -0600
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Re: Etching plastic for T blue staining
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000101c9e865$b39f1ce0$1add56a0$@callis <@t> bresnan.net>
Content-Type: text/plain;	charset="us-ascii"

You wrote: 

Chris,  If you do not do this, consider etching your tissue to remove the
surface plastic. We use 0.2% formic acid - start with less than a minute as
your tissues are thin.  Some labs etch using ethyl alcohol or methanol.  We
have success.  

Using the formic acid is commonly done on undecalcified bone embedded in
methyl methacrylate to achieve a mild surface decalcification of the bone.
This removes a few micrometers of calcium from bone in thicker slab section
(ground and polished) and on thinner microtomed MMA sections.  However, I
don't think the acid actually dissolves/removes plastic itself.  This is why
acid etched plastic bone permit certain low molecular weight dyes (toludine
blue, basic fuchsin, methylene blue and dye mixtures e.g. Sanderson's rapid
bone stain and Shenks recipe MacNeals tertrachrome) to penetrate into the
bone matrix of thicker slabs.  

 However, etching the plastic with alcohols is a good way to soften and
possibly remove some of the plastic to allow better penetration of dyes into
the soft tissues.  Glycol methacrylate is not removable, MMA is removable
using xylene and some other solvents, and if you use an EM resin, you may
have to do sodium ethoxide treatment to remove plastic.  For EM resin
embedded sections at 1 um or so, we uses  Toluidine blue/sodium borate , pH
11, by flooding a section then heating on a hot plate, rinsing, drying and
coverslippling.   Most of the time, tissues embedded in GMA or MMA will
stain with a toluidine blue method, particularly when the pH is 8 or higher.

There is a very good discussion on the etching, dyes, pH and temperature
effects on various plastics in this publication.  Horobin RW.  Staining
plastic sections:  a review of problems, explanations, and possible
solutions.  J Microscopy 131:173-186, 1982.   

Gayle M. Callis

HTL(ASCP)HT,MT

Bozeman MT 59715

------------------------------

Message: 5
Date: Mon, 8 Jun 2009 12:46:51 -0600
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Quenching autofluorescence
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000501c9e869$7df15d70$79d41850$@callis <@t> bresnan.net>
Content-Type: text/plain;	charset="us-ascii"

You wrote:  I'm wondering how to quench auto-fluorescence in tissue from a
mouse that was treated with a FITC-labeled protein.  What do people usually
use to quench the endogenous auto-fluorescence, and will this treatment

quench the FITC signal from the labeled protein?

Jennifer M. Anderson, Scientist

Halozyme Therapeutics, Inc.

11388 Sorrento Valley Road

San Diego, CA 92121

858-704-8333

First, you didn't say how the tissues were treated after removal from
animal?  Are you doing FFPE or frozen sections, etc???? Was this an injected
protein-FITC? More details will help. 

  A method to remove auto-fluorescence will depend on the type of
auto-fluorescence you experience due to the fact there are more than one
source of this problem. An excellent discussion with methods for removal are
found on the Wright Cell Imaging Facility website.
http://www.uhnres.utoronto.ca/facilities/wcif/PDF/Autofluorescence.pdf    

Good luck

Gayle M. Callis

HTL(ASCP)HT,MT

Bozeman MT 59715   

------------------------------

Message: 6
Date: Mon, 8 Jun 2009 14:51:21 -0400
From: "Goodwin, Diana" <GoodwinD <@t> pahosp.com>
Subject: [Histonet] Preservation/transport medium for tissue culture
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0C4A1 <@t> uphsmbx2.UPHS.PENNHEALTH.PRV>

Content-Type: text/plain;	charset="US-ASCII"

Greetings.

My knowledge of tissue culture is non-existent.  What is the recommended
preservative/transport medium for tissue (namely foreskin) to be used
for tissue culture that has a viability factor of 1-2 weeks?  Also need
the storage requirements.

Thanks in advance!

Diana Goodwin
Supervisor, Anatomic Pathology
Pennsylvania Hospital
Preston 655-C
ph. 215-829-6532
pager 215-422-5160
fax 215-829-7564
e-mail goodwind@ pahosp.com

The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.

------------------------------

Message: 7
Date: Mon, 8 Jun 2009 12:48:14 -0700 (PDT)
From: emerald_lake77 <@t> yahoo.com
Subject: [Histonet] Low pH HIER -- Tris-HCl buffer
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <382303.66672.qm <@t> web110603.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello all,

Has anyone ever tried using a Tris buffer solution at very low pH (pH 1 or 2) to retrieve epitopes whether through a microwave, steamer or pressure cooker?  I am trying to retrieve 10%NBF, 24-48 hr fixed mouse and rat tissue for an antigen that papers and books suggest should be retrieved using low pH Tris buffer.

I was just wondering if anyone has had any experience(s) to share ... buffer solution recipes and subsequent positive or negative (e.g. poor) results.

Thank you.

Gustave

Gustave T. Hebert
Research Scientist I
Metabolic Disease Research
Wyeth Research
200 CambridgePark Drive

Keywords: low ph HIER, retrieval, retreival, epitope, antibody, tris, tris-hcl, buffer

------------------------------

Message: 8
Date: Mon, 8 Jun 2009 17:50:58 -0400
From: "Lena Spencer" <lenaspencer <@t> insightbb.com>
Subject: [Histonet] FW: new reference books
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001801c9e883$354a9590$9fdfc0b0$@com>
Content-Type: text/plain;	charset="us-ascii"

From: Lena Spencer [mailto:lenaspencer <@t> insightbb.com] 
Sent: Sunday, June 07, 2009 7:45 PM
To: 'histonet-request <@t> lists.utsouthwestern.edu'
Subject: new reference books

Hi All:

I know that many of you have been looking for new reference books.  There
are now two new ones on the market.  Freida Carson's, 3rd edition is hot off
the ASCP press and available for purchase from the ASCP go to the webpage
and an order, www.ascp.org .

'0Histologic Preparations Common Problems and Their Solutions" is an Atlas
that covers most common special stains, processing and microtomy problem.
This book has pictures of good and bad examples of stains, there is a
discussion about each stain, controls,  problems encounter and possible
solutions, references and so much more.  There will be a sample chapter (or
part of a chapter) in CAP Today.  The Atlas is written by NSH Members who
served on the HistoQIP Committee and Edited by Dr.  Richard Brown, who
served as Chairman of the committee.  The Atlas can be purchased on the CAP
webpage - www.cap.org

Hopefully you will find these two books great references for your laboratory
and your personal library.

Lena Spencer    

------------------------------

Message: 9
Date: Tue, 9 Jun 2009 09:28:54 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: RE: [Histonet] Storage of tissues in PFA
	beforedehydrationandembedding
To: <tifei <@t> foxmail.com>, "Patsy Ruegg" <pruegg <@t> ihctech.net>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB037D <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="gb2312"

Then you will have to use frozen sections not FFPE tissues.
May be one of the formalin-free fixatives might work.
You will need to experiment and see

Regards 

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

	-----Original Message-----
	From: TF [mailto:tifei <@t> foxmail.com] 
	Sent: Monday, 8 June 2009 12:50 PM
	To: Patsy Ruegg; Tony Henwood; histonet <@t> lists.utsouthwestern.edu
	Subject: Re: RE: [Histonet] Storage of tissues in PFA beforedehydrationandembedding

	Just want to mention a point that some antigens are too fragile to PFA and in my hand can not be retrieval"ed" anymore...such as rat CD31.

	2009-06-08 

________________________________

	TF 

________________________________

	·¢¼þÈË£º Patsy Ruegg 
	·¢ËÍÊ±¼ä£º 2009-06-08  00:13:46 
	ÊÕ¼þÈË£º 'Tony Henwood'; histonet <@t> lists.utsouthwestern.edu 
	³ËÍ£º 
	Ö÷Ìâ£º RE: [Histonet] Storage of tissues in PFA beforedehydrationandembedding 

	I agree with Tony, the problem is usually underfixation not over with
	aldehyde fixatives, if you do not stabilize the proteins by fixing (Bryan
	Hewlett says this takes 24 hrs. no matter the size of the tissue) paraffin
	processing will adversely affect your tissue and in some cases the proteins
	of interest will be washed away and lost with no change or no amount of AR
	to get them back.  Researchers are stuck in the days before AR techniques
	when cross linking of proteins by aldehyde fixation was a problem because we
	did not know how to retrieve them, but now we do, and the bigger problem is
	losing antigens from paraffin processing because they have not been
	adequately fixed to protect them.  I recommend 24-72 hour fixation in
	aldehyde fixative and then the tissues can be placed in 70% alcohol.
	Patsy
	Patsy Ruegg, HT(ASCP)QIHC
	IHCtech
	12635 Montview Blvd. Ste.215
	Aurora, CO 80045
	720-859-4060
	fax 720-859-4110
	www.ihctech.net 
	www.ihcrg.org
	-----Original Message-----
	From: histonet-bounces <@t> lists.utsouthwestern.edu
	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood
	Sent: Thursday, June 04, 2009 5:22 PM
	To: histonet <@t> lists.utsouthwestern.edu
	Subject: RE: [Histonet] Storage of tissues in PFA before
	dehydrationandembedding
	Nick,
	Well after 30 years of doing ICCs, the worst results are nearly always
	with tissues that have not been adequately fixed in formalin. This is
	based on using over 300 different antibodies in both adult and pediatric
	settings.
	The following paper shows an example of what can go wrong with
	under-fixed tissue:
	Gomes L, Mackie N, Catchpoole D, Henwood T (2008) "Test Your Knowledge"
	J Histotechnol 31(4):138-184
	Regards
	Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
	Manager & Senior Scientist
	Tel: 612 9845 3306
	Fax: 612 9845 3318
	the children's hospital at westmead 
	Cnr Hawkesbury Road and Hainsworth Street, Westmead 
	Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 
	-----Original Message-----
	From: Nicholas David Evans [mailto:ndevans <@t> stanford.edu] 
	Sent: Friday, 5 June 2009 8:56 AM
	To: Tony Henwood
	Subject: RE: [Histonet] Storage of tissues in PFA before dehydration
	andembedding
	Thanks for the helpful response. 
	After a consensus of views I decided to store at 70% ethanol for several
	days following 3-4hrs in 4% PFA. I think many people use paraffin
	embedded tissues for ICC and over-fixation is deleterious for this. 4%
	Paraformaldehyde (PFA) = 4g PFA dissolved in 100 mL PBS. Indeed when in
	solution it is present as formaldehyde. I will consider your suggestion
	of frozen sections if I have poor results.
	Thanks again for the e mail and best wishes
	Nick
	-----Original Message-----
	From: Tony Henwood [mailto:AnthonyH <@t> chw.edu.au] 
	Sent: 03 June 2009 16:54
	To: Nicholas David Evans; histonet <@t> lists.utsouthwestern.edu
	Subject: RE: [Histonet] Storage of tissues in PFA before dehydration
	andembedding
	Nick,
	Where did you hear that "storage in PFA for longer than 24 hrs may
	adversely affect my tissues"? The beauty of 4% formaldehyde (especially
	if it is buffered) is that it does not adversely affect tissues, in fact
	it protects them brilliantly from the rigours of subsequent processing.
	If it is immunohistochemistry using formalin-labile antigens that you
	are worried about then use frozen sections not FFPE sections.
	The major cause of poor morphology in FFPE tissues is inadequate
	formalin fixation. If you want ethanol fixed tissues (and rotten
	morphology) then leave out the formalin fixation step altogether.
	AND how do you fix it in 4% polyformaldehyde? Surely when it is in
	solution it becomes formaldehyde?
	Regards
	Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
	Manager & Senior Scientist
	Tel: 612 9845 3306
	Fax: 612 9845 3318
	the children's hospital at westmead
	Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,
	Westmead NSW 2145, AUSTRALIA 
	-----Original Message-----
	From: histonet-bounces <@t> lists.utsouthwestern.edu
	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nicholas
	David Evans
	Sent: Thursday, 4 June 2009 3:59 AM
	To: histonet <@t> lists.utsouthwestern.edu
	Subject: [Histonet] Storage of tissues in PFA before dehydration
	andembedding
	Dear all,

	I am doing some experiments where mouse skin tissue is harvested for
	immuno and in situ staining at 2, 4, 6 and 8 days following a treatment.
	The usual protocol used in our lab (for other tissues, usually bone) is
	to fix overnight in 4% PFA before dehydration and paraffin embedding. As
	I value my weekends, I would obviously prefer to do the dehydration
	(which we do in ethanol series, an hour in each of 30, 50, 70, 90, 95,
	100% ethanol = 6 hours, before overnight in 100%) and embedding of all
	samples on the same day. 

	I understand that storage in PFA for longer than 24 hrs may adversely
	affect my tissues. Is it possible to store for longer in a lower
	concentration of PFA, or in another buffer until I am ready to dehydrate
	and embed? In short, I would appreciate if anyone could suggest a way to
	plan this experiment in the most efficient way. 

	With thanks and best wishes
	Nick Evans

	Dept Surgery
	Stanford University _______________________________________________
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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

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------------------------------

Message: 10
Date: Tue, 9 Jun 2009 09:34:23 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Preservation/transport medium for tissue
	culture
To: "Goodwin, Diana" <GoodwinD <@t> pahosp.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB037E <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Diana,

I would use Hank's solution.
Sigma Cat No.H9269-100ml

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Goodwin,
Diana
Sent: Tuesday, 9 June 2009 4:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Preservation/transport medium for tissue culture

Greetings.

My knowledge of tissue culture is non-existent.  What is the recommended
preservative/transport medium for tissue (namely foreskin) to be used
for tissue culture that has a viability factor of 1-2 weeks?  Also need
the storage requirements.

Thanks in advance!

Diana Goodwin
Supervisor, Anatomic Pathology
Pennsylvania Hospital
Preston 655-C
ph. 215-829-6532
pager 215-422-5160
fax 215-829-7564
e-mail goodwind@ pahosp.com

The information contained in this e-mail message is intended only for
the personal and confidential use of the recipient(s) named above. If
the reader of this message is not the intended recipient or an agent
responsible for delivering it to the intended recipient, you are hereby
notified that you have received this document in error and that any
review, dissemination, distribution, or copying of this message is
strictly prohibited. If you have received this communication in error,
please notify us immediately by e-mail, and delete the original message.
_______________________________________________
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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************

------------------------------

Message: 11
Date: Tue, 9 Jun 2009 11:36:16 +0100
From: "Hobbs, Carl" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] Re: MAP2 and GFAP double staining
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<11D9615B89C10747B1C985966A63D7CA2961FE0EA3 <@t> KCL-MAIL04.kclad.ds.kcl.ac.uk>

Content-Type: text/plain; charset="iso-8859-1"

Antibodies
MAP2 Sigma M4403
GFAP Dako Z0334
You don't tell what application nor species, nor any detection details.
I have not done double-labelling with these two but they individually work very, very well in frozen/pwax sections of human, mouse and rat so they will be fine for double-labelling.
carl

------------------------------

Message: 12
Date: Tue, 9 Jun 2009 10:13:45 -0400
From: "Melissa Ribeiro" <melissa.ribeiro <@t> brinegroup.com>
Subject: [Histonet] Histology Manager opportunity in Connecticut 
To: <histonet <@t> lists.utsouthwestern.edu>,
	<histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID:
	<43904A2EECEAB54D8A023931049FEA4C12AA9B <@t> brin-sbs01.brinegroup.local>
Content-Type: text/plain;	charset="us-ascii"

Brine Group conducting a search for a HISTOLOGY MANAGER

in a community hospital based clinical lab in Southeastern Connecticut.

Looking for strong leadership skills, a vision of future technologies
for Histology, 

and ability to interact with pathologists to keep the section on the
"cutting edge".

MAJOR ACCOUNTABILITIES/CRITICAL RESPONSIBILITIES 

Responsible for daily operations within the Histology department: 

*                     Manages and supervises Histology staff ensuring
the department runs effectively. 

*                     Responsible for maintaining and revising Histology
policies and procedures. 

*                     Responsible for developing and monitoring section
budget. Reviews budget monthly for variances and eliminating negative 

variances. 

*                     Hires qualified staff, disciplines and terminate
staff as appropriate in a consistent and timely fashion. 

*                     Conducts staff meetings, keeping staff informed
about departmental and hospital wide information. 

*                     Ensure staff competency by assessing educational
and developmental needs of the staff, developing and maintaining 

competency program and completes annual performance reviews in a timely
fashion. 

*                     Effectively troubleshoots and resolve technical
and clinical issues. 

*                     Develops and monitors Quality Indicators.
Identifies trends and acts on opportunities for improvement. 

*                     Ensure adequate staffing for department and
coverage for all shifts. 

*                     Ensures compliance with all Laboratory and
Hospital safety policies as well as other Hospital policies. 

*                     Responsible for department QC procedures and
troubleshoots QC failures with proper documentation. 

*                     Prepares and monitors daily/weekly/monthly quality
control for Histology. 

*                     Participates in inter and intra-department QA
initiatives. 

*                     Responds to technical issues from physicians and
works to appropriately resolve them. 

*                     Maintains technical competency and keeps current
on new testing available for Histology. 

*                     Acts as a resource person for new Histology
testing/instrumentation. 

*                     Effectively utilizes all necessary LIS functions. 

*                     Maintains appropriate levels of inventory. 

*                     Responsible for Histology in all Laboratory and
Hospital inspections; CAP, JCAHO and the State of Connecticut.

QUALIFICATIONS/REQUIREMENTS

Education:       Minimum B.S. degree in Laboratory Science. Prefer
graduate of formal Histology training program.

Experience:     Working knowledge of gross human anatomy required. 

Five years of Histology experience with a superior performance record
required. 

Previous supervisory experience preferred.

Knowledgeable of current and new technologies in Histology.

Training:          Histologic Technique required.

Licensure:       ASCP certification preferred.

All interested/qualified candidates please contact Melissa Ribeiro 

at (781) 272-3400 ext. 228 or via e-mail at
<mailto:mribeiro <@t> brinegroup.com> mribeiro <@t> brinegroup.com
<mailto:mribeiro <@t> brinegroup.com?subject=Histology%20Manager%20-%20Connec
ticut> 

Melissa Ribeiro

Healthcare Division

Brine Group Staffing Solutions

20 Mall Road, Suite 225

Burlington, MA 01803

<mailto:mribeiro <@t> brinegroup.com> mribeiro <@t> brinegroup.com
<mailto:mribeiro <@t> brinegroup.com?subject=Histology%20Manager%20-%20Connec
ticut> 

Ph.  (781) 272-3400 ext. 228

Fax (781) 494-3401

------------------------------

Message: 13
Date: Tue, 9 Jun 2009 08:38:24 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: [Histonet] Gram Stain
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
	<57BE698966D5C54EAE8612E8941D768305CE8209 <@t> EXCHANGE3.huntingtonhospital.com>

Content-Type: text/plain;	charset="us-ascii"

Can anyone recommend a good gram stain kit?  We currently do a modified
Brown-Hopps stain and make up all of the reagents, but I am looking for
a kit.

Laurie Colbert

------------------------------

Message: 14
Date: Tue, 9 Jun 2009 18:49:20 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: [Histonet] antibody recommendation
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <128F56C6DCDE4207937936DBE9A007FC <@t> dielangs.at>
Content-Type: text/plain;	charset="us-ascii"

Hi!

Can anyone help me with a recommendation for CDK4 and MDM2 antibody for
human FFPE tissue?

We use the Benchmark XT as immunhisto-instrument.

Thanks in advance

Gudrun Lang

------------------------------

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End of Histonet Digest, Vol 67, Issue 9
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