[Histonet] Low pH HIER -- Tris-HCl buffer

emerald_lake77 <@t> yahoo.com emerald_lake77 <@t> yahoo.com
Mon Jun 8 14:48:14 CDT 2009

Hello all,
Has anyone ever tried using a Tris buffer solution at very low pH (pH 1 or 2) to retrieve epitopes whether through a microwave, steamer or pressure cooker?  I am trying to retrieve 10%NBF, 24-48 hr fixed mouse and rat tissue for an antigen that papers and books suggest should be retrieved using low pH Tris buffer.
I was just wondering if anyone has had any experience(s) to share ... buffer solution recipes and subsequent positive or negative (e.g. poor) results.
Thank you.
Gustave T. Hebert
Research Scientist I
Metabolic Disease Research
Wyeth Research
200 CambridgePark Drive
Keywords: low ph HIER, retrieval, retreival, epitope, antibody, tris, tris-hcl, buffer


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