[Histonet] Quenching autofluorescence

[gayle callis]

You wrote:  I'm wondering how to quench auto-fluorescence in tissue from a
mouse that was treated with a FITC-labeled protein.  What do people usually
use to quench the endogenous auto-fluorescence, and will this treatment

quench the FITC signal from the labeled protein?

 

Jennifer M. Anderson, Scientist

Halozyme Therapeutics, Inc.

11388 Sorrento Valley Road

San Diego, CA 92121

858-704-8333

 

First, you didn't say how the tissues were treated after removal from
animal?  Are you doing FFPE or frozen sections, etc???? Was this an injected
protein-FITC? More details will help. 

 

  A method to remove auto-fluorescence will depend on the type of
auto-fluorescence you experience due to the fact there are more than one
source of this problem. An excellent discussion with methods for removal are
found on the Wright Cell Imaging Facility website.
http://www.uhnres.utoronto.ca/facilities/wcif/PDF/Autofluorescence.pdf    

 

Good luck

 

Gayle M. Callis

HTL(ASCP)HT,MT

Bozeman MT 59715