[Histonet] Quenching autofluorescence
gayle.callis <@t> bresnan.net
Mon Jun 8 13:46:51 CDT 2009
You wrote: I'm wondering how to quench auto-fluorescence in tissue from a
mouse that was treated with a FITC-labeled protein. What do people usually
use to quench the endogenous auto-fluorescence, and will this treatment
quench the FITC signal from the labeled protein?
Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
First, you didn't say how the tissues were treated after removal from
animal? Are you doing FFPE or frozen sections, etc???? Was this an injected
protein-FITC? More details will help.
A method to remove auto-fluorescence will depend on the type of
auto-fluorescence you experience due to the fact there are more than one
source of this problem. An excellent discussion with methods for removal are
found on the Wright Cell Imaging Facility website.
Gayle M. Callis
Bozeman MT 59715
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