[Histonet] Frozen Artifacts

[Va Paula Sicurello]

Hi Nicole,

You must tell the list what the artifacts are before we can help you.

You're note is a little too vague.

Paula Sicurello

VA Medical Center San Diego

Veterans Medical Research Foundation (VMRF) 

Core Research Imaging Center (CRIC)

3350 La Jolla Village Dr., MC151

San Diego, CA 92161

858-552-8585 x2397



Your images flow through our CRIC.

--- On Fri, 6/5/09, Patten, Nicole (NIH/NIAAA) [F] <pattennj <@t> mail.nih.gov> wrote:

From: Patten, Nicole (NIH/NIAAA) [F] <pattennj <@t> mail.nih.gov>
Subject: [Histonet] Frozen Artifacts
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Cc: "Rodriguez-canales, Jaime (NIH/NCI) [F]" <rodrigja <@t> mail.nih.gov>
Date: Friday, June 5, 2009, 7:46 PM

Hello Histonet...

I am using frozen brain sections and I have terrible frozen artifacts. I receive the tissue from a brain bank so I have no control over the method of freezing. Is there ANYTHING that I can do at this point to at least reduce these artifacts? I've tried formalin fixation post-cutting and reducing the temperature of the cryostat but nothing works.... the others in my lab also have no idea. Any help would be GREATLY appreciated. Thanks!!

-Nicole

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