[Histonet] Peroxidase block again................

[Tony Henwood]

Gene,
A few references that might be of use:

Jylling et al "Immunohistochemistry on Frozen Section of Sentinel Lymph
Nodes in Breast Cancer With Improved Morphology and Blocking of
Endogenous Peroxidase" Appl Immunohistochem Mol Morphol 2008;16:482-484
- Simultaneous blocking of endogenous peroxidase and antigen retrieval
was carried out by microwaving at 1000W in TRIS-EGTA buffer, pH 9,0
preheated to 601C until boiling point was reached (approximately 30 s).

And:

Gao et al "Microwave Antigen Retrieval Blocks Endogenous Peroxidase
Activity in Immunohistochemistry" Appl Immunohistochem Mol Morphol
2008;16:393-399 - suggesting that microwave exposure (HIER) is able to
block the endogenous peroxidase.



Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: njoydobro <@t> aol.com [mailto:njoydobro <@t> aol.com] 
Sent: Monday, 1 June 2009 6:59 PM
To: Tony Henwood
Subject: Re: [Histonet] Peroxidase block again................


Can't use it.





-----Original Message-----
From: Tony Henwood <AnthonyH <@t> chw.edu.au>
To: njoydobro <@t> aol.com; Histonet <@t> lists.utsouthwestern.edu
Sent: Sun, 31 May 2009 7:51 pm
Subject: RE: [Histonet] Peroxidase block again................


Yep,

You can use alkaline phosphatase labelling instead of peroxidase

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
njoydobro <@t> aol.com
Sent: Friday, 29 May 2009 9:20 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Peroxidase block again................


good morning,

???? I would like to pose this questions again...Is there anyone out
there who has eliminated using Peroxidase Block on either IHC or ICC?
Your feedback is very helpful.


Thanks,
Gene
Cleveland Clinic _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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