[Histonet] RE: CD11c for murine tissu

[Patsy Ruegg]

I beg your pardon, you are correct, I was thinking of cd11b not cd11c, as
F4/80 is for macs not dendritic cells.

Best regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: anh2006 <@t> med.cornell.edu [mailto:anh2006 <@t> med.cornell.edu] 
Sent: Monday, June 01, 2009 7:51 AM
To: Patsy Ruegg; histonet-bounces <@t> lists.utsouthwestern.edu; 'gayle callis';
'Histonet'
Subject: Re: [Histonet] RE: CD11c for murine tissu

Correct me if I am wrong but CD11c is predominantly on dendritic cells and
F4/80 is for predominantly macrophage/myeloid populations (therefore a good
CD11b substitute). Are you sure you should use F4/80 as a CD11c substitute? 


-----Original Message-----
From: Patsy Ruegg <pruegg <@t> ihctech.net>

Date: Sat, 30 May 2009 10:44:14 
To: 'gayle callis'<gayle.callis <@t> bresnan.net>;
'Histonet'<histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] RE: CD11c for murine tissu


An alternative to cd11c for macrophages is rat anti mouse f4/80 which I use
from Serotec, it works in frozen and ffpe tissue.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Saturday, May 30, 2009 8:45 AM
To: 'Histonet'
Subject: [Histonet] RE: CD11c for murine tissu

You wrote:  Hi all - I'm looking for a good antibody to use on mouse tissue
(frozen) 

to look for CD11c - does anyone have a suggestion for a good antibody 

source? Thanks in advance - Melissa

 

We us CD11c, HL3 clone, Armenian Hamster anti Mouse.  Negative control is
Armenian Hamster IgG1 from BD Pharmingen/Invitrogen.  Tissues are fresh
frozen sections fixed with our favorite acetone/absolute ethanol fixative,
although you can use 4C acetone for 10 min on overnight air dried sections.
I will be happy to send the other fixative recipe if you want it. 

 

Gayle M. Callis

HTL(ASCP)HT,MT

Bozeman MT 

 

 

 

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