[Histonet] Problems with IHC

[Mike Valerio]

Hello,
I am having problems staining my FFPE mouse brains for macrophages.  I started out with F4/80 
marker, but have now moved to CD68.  My positive controls (mouse spleen and liver) work fine, but 
I cannot get anything to stain in my sample tissues.  I have see through H and E staining that 
macrophages are present, could anyone help me with this problem?
Mike Valerio
University of Buffalo