[Histonet] RE: Histonet Digest, Vol 68, Issue 29

Barone, Carol cbarone <@t> NEMOURS.ORG
Thu Jul 23 16:00:44 CDT 2009


The discussion on the 70's has been very nostalgic. I also worked in a lab with an Auto-technicon, no hood, benezene and chloroform, mixed with the smell of xylene and formalin (it was the aroma therapy, of the decade for histology). We didn't use gloves to reach into the open beaker to fetch out the cassettes and plastics were done in the adjoining lab, no hood, no door. All reagents were just stored under the table...in pretty red containers and the dust from preparing fresh dyes, often dusted the open balance. My pathologist smoked in the morgue during the autopsies... and I wish I only knew then, what I know now.

However, at age 61, people often compliment me on how good my skin looks. I usually reply...aroma therapy and good fixation!

PS: My lab is now LEAN and Green and my tech's use all PPE. Safety in the Lab is priority one! ...and a final note, I work on an entirely smoke free campus! How the times have changed!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, July 23, 2009 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68, Issue 29


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Today's Topics:

   1. RE: Start Up Lab (Edwards, R.E.)
   2. Rat's lung (tahseen <@t> brain.net.pk)
   3. RE: Start Up Lab (Lynette Pavelich)
   4. RE: Start Up Lab (Bartlett, Jeanine (CDC/CCID/NCZVED))
   5. RE: Start Up Lab (Smith, Allen)
   6. RE: Start Up Lab (Bartlett, Jeanine (CDC/CCID/NCZVED))
   7. RE: Peloris tissue processor  (Joyce Cline)


----------------------------------------------------------------------

Message: 1
Date: Thu, 23 Jul 2009 16:51:05 +0100
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: RE: [Histonet] Start Up Lab
To: "'Bartlett, Jeanine (CDC/CCID/NCZVED)'" <jqb7 <@t> cdc.gov>, Montina
	Van Meter	<Montina.VanMeter <@t> pbrc.edu>, Carol Bryant
	<cbrya <@t> lexclin.com>,	"thecitan <@t> yahoo.com" <thecitan <@t> yahoo.com>,
	Lynette Pavelich	<lpaveli1 <@t> hurleymc.com>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<7722595275A4DD4FA225B92CDBF174A18C7DAA9B3D <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"

You still  have  fingers!?.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: 23 July 2009 16:44
To: Montina Van Meter; Carol Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their "personal drawer".  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thecitan <@t> yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-----Original Message-----
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>

Date: Wed, 22 Jul 2009 18:07:07
To: <leiker <@t> buffalo.edu>; <histonet <@t> lists.utsouthwestern.edu>;
<CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Start Up Lab


Gosh.........I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh......

>>> Merced M Leiker <leiker <@t> buffalo.edu> 07/22/09 5:00 PM >>>
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
<CIngles <@t> uwhealth.org> wrote:

> In the lab?!? For shame. :)
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Cindy
DuBois
> Sent: Wed 7/22/2009 10:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Start Up Lab
>
>
>
> And a coffee pot.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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------------------------------

Message: 2
Date: Thu, 23 Jul 2009 21:51:16 +0600 (PKST)
From: tahseen <@t> brain.net.pk
Subject: [Histonet] Rat's lung
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <63947.202.125.145.178.1248364276.squirrel <@t> brain.net.pk>
Content-Type: text/plain;charset=iso-8859-1

Dear All,
How the volume of Rat's lung can be meausured?
Thanks advance
Muhammad Tahseen
Histology Supervisor
SKMCH&RC
lAHORE,pAKISTAN





------------------------------

Message: 3
Date: Thu, 23 Jul 2009 11:53:27 -0400
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>
Subject: RE: [Histonet] Start Up Lab
To: <cbrya <@t> lexclin.com>,<Montina.VanMeter <@t> pbrc.edu>,
	<thecitan <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4A684F37020000EE0002AFD3 <@t> smtp-gw.hurleymc.com>
Content-Type: text/plain; charset=US-ASCII

Ah, yes.  The '70's.  Our lab had 2 windows, both on the same side of
the wall.  The pathologists cut under one of the windows and it had one
of those box fans, pointing out.  In Michigan.  No hoods, an old
duo-autotechnicon and xylene in full use with disposal down the drain. 
Every day, I would go home not knowing if I went thru a red light or
not, arriving home , head still cloudy for about 30 minutes until it
cleared.  Ah..those were the days??  Although I miss it, I'm glad I had
to give up my coffee and donut when cutting.  Treats in the drawer are
gone, mouth pipetting is gone (not mine, but yes, the mouth does turn
black from silver nitrate!!)  Progress is good.  But I still like my
'70's music!!!  Bob Seger and old motown!!! ;)

>>> "Montina Van Meter" <Montina.VanMeter <@t> pbrc.edu> 07/23/09 11:31 AM
>>>
I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their "personal drawer".  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thecitan <@t> yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>

Date: Wed, 22 Jul 2009 18:07:07 
To: <leiker <@t> buffalo.edu>; <histonet <@t> lists.utsouthwestern.edu>;
<CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Start Up Lab


Gosh.........I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh......

>>> Merced M Leiker <leiker <@t> buffalo.edu> 07/22/09 5:00 PM >>>
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
<CIngles <@t> uwhealth.org> wrote:

> In the lab?!? For shame. :)
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Cindy
DuBois
> Sent: Wed 7/22/2009 10:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Start Up Lab
>
>
>
> And a coffee pot.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
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------------------------------

Message: 4
Date: Thu, 23 Jul 2009 12:13:19 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Start Up Lab
To: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>,	"Montina Van Meter"
	<Montina.VanMeter <@t> pbrc.edu>,	"Carol Bryant" <cbrya <@t> lexclin.com>,
	thecitan <@t> yahoo.com,	"Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<9A16CB5D55FC1648ADF11B63E72A1BE101AD3400 <@t> LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii

Hah!  Sure do..... 




-----Original Message-----
From: Edwards, R.E. [mailto:ree3 <@t> leicester.ac.uk] 
Sent: Thursday, July 23, 2009 11:51 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol
Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

You still  have  fingers!?.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: 23 July 2009 16:44
To: Montina Van Meter; Carol Bryant; thecitan <@t> yahoo.com; Lynette
Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their "personal drawer".  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thecitan <@t> yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-----Original Message-----
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>

Date: Wed, 22 Jul 2009 18:07:07
To: <leiker <@t> buffalo.edu>; <histonet <@t> lists.utsouthwestern.edu>;
<CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Start Up Lab


Gosh.........I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh......

>>> Merced M Leiker <leiker <@t> buffalo.edu> 07/22/09 5:00 PM >>>
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
<CIngles <@t> uwhealth.org> wrote:

> In the lab?!? For shame. :)
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Cindy
DuBois
> Sent: Wed 7/22/2009 10:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Start Up Lab
>
>
>
> And a coffee pot.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
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_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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------------------------------

Message: 5
Date: Thu, 23 Jul 2009 12:26:26 -0400
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Start Up Lab
To: "'Bartlett, Jeanine (CDC/CCID/NCZVED)'" <jqb7 <@t> cdc.gov>
Cc: "'Histonet <@t> lists.utsouthwestern.edu'"
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E4132130AC2F764D8C173C5400D53042A73790012E <@t> exchsrv02.barrynet.barry.edu>
	
Content-Type: text/plain; charset="us-ascii"

I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent.  I think you mean dioxane.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, July 23, 2009 12:13 PM
To: Edwards, R.E.; Montina Van Meter; Carol Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Hah!  Sure do..... 




-----Original Message-----
From: Edwards, R.E. [mailto:ree3 <@t> leicester.ac.uk] 
Sent: Thursday, July 23, 2009 11:51 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol
Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

You still  have  fingers!?.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: 23 July 2009 16:44
To: Montina Van Meter; Carol Bryant; thecitan <@t> yahoo.com; Lynette
Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their "personal drawer".  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thecitan <@t> yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-----Original Message-----
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>

Date: Wed, 22 Jul 2009 18:07:07
To: <leiker <@t> buffalo.edu>; <histonet <@t> lists.utsouthwestern.edu>;
<CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Start Up Lab


Gosh.........I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh......

>>> Merced M Leiker <leiker <@t> buffalo.edu> 07/22/09 5:00 PM >>>
(lol some labs have a bench area as well as a desk area where food is 
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire 
<CIngles <@t> uwhealth.org> wrote:

> In the lab?!? For shame. :)
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Cindy
DuBois
> Sent: Wed 7/22/2009 10:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Start Up Lab
>
>
>
> And a coffee pot.
>
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>
> _______________________________________________
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Message: 6
Date: Thu, 23 Jul 2009 12:32:07 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Start Up Lab
To: "Smith, Allen" <asmith <@t> mail.barry.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<9A16CB5D55FC1648ADF11B63E72A1BE101AD3402 <@t> LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii

Thanks to spell-check......it self-corrected me......incorrectly!
Thanks for setting it right.



-----Original Message-----
From: Smith, Allen [mailto:asmith <@t> mail.barry.edu] 
Sent: Thursday, July 23, 2009 12:26 PM
To: Bartlett, Jeanine (CDC/CCID/NCZVED)
Cc: 'Histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Start Up Lab

I can't imagine why any one would use dioxin, which used to be used as
an insulator and heat convector in electron microscope transformers, as
a clearing agent.  I think you mean dioxane.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, July 23, 2009 12:13 PM
To: Edwards, R.E.; Montina Van Meter; Carol Bryant; thecitan <@t> yahoo.com;
Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Hah!  Sure do..... 




-----Original Message-----
From: Edwards, R.E. [mailto:ree3 <@t> leicester.ac.uk]
Sent: Thursday, July 23, 2009 11:51 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol
Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

You still  have  fingers!?.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: 23 July 2009 16:44
To: Montina Van Meter; Carol Bryant; thecitan <@t> yahoo.com; Lynette
Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

And I used to use dioxin in my tissue processor and would actually dip
gauze in it to clean paraffin off my fingers. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Montina
Van Meter
Sent: Thursday, July 23, 2009 11:31 AM
To: Carol Bryant; thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on  cigarettes with an
ashtray perched on top of her microtome.  When we had inspections
everyone would put their contraband in their "personal drawer".  I also
interned in an ENT lab that processed with celloidin (=ETHER) and didn't
have windows or a hood.  That supervisor also smoked in the lab!  I said
a prayer every day that I wouldn't blow up or be asphyxiated during my
two week stay.  


 

Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Thursday, July 23, 2009 9:04 AM
To: thecitan <@t> yahoo.com; Lynette Pavelich
Cc: Histonet
Subject: RE: [Histonet] Start Up Lab

Love it!  Too funny! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thecitan <@t> yahoo.com
Sent: Wednesday, July 22, 2009 6:59 PM
To: Lynette Pavelich
Cc: Histonet
Subject: Re: [Histonet] Start Up Lab

Dang maybe I should stop keeping my lunch in the cryostat. The fresh
unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent
from my Verizon Wireless BlackBerry

-----Original Message-----
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>

Date: Wed, 22 Jul 2009 18:07:07
To: <leiker <@t> buffalo.edu>; <histonet <@t> lists.utsouthwestern.edu>;
<CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Start Up Lab


Gosh.........I remember the days sipping on my coffee and nibbling on a
fresh donut as I cut my morning slides!  Sigh......

>>> Merced M Leiker <leiker <@t> buffalo.edu> 07/22/09 5:00 PM >>>
(lol some labs have a bench area as well as a desk area where food is
allowed.)

--On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire
<CIngles <@t> uwhealth.org> wrote:

> In the lab?!? For shame. :)
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Cindy
DuBois
> Sent: Wed 7/22/2009 10:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Start Up Lab
>
>
>
> And a coffee pot.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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------------------------------

Message: 7
Date: Thu, 23 Jul 2009 12:47:52 -0400
From: "Joyce Cline" <jcline <@t> wchsys.org>
Subject: RE: [Histonet] Peloris tissue processor 
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <65281717443C4C1385063D050EB7A387 <@t> wchsys.org>
Content-Type: text/plain;	charset="us-ascii"

We have had our Peloris since Feb. and we love it. We use the 6 hour program
for our regular tissue and the 8 hour program for breast, thyroid, colon,
melanoma, sentinel nodes, prostatectomy. The one hour program for prostate
biopsies. We use Formula 83 instead of Xylene, or you can use Xylene free
processing using Isopropyl alcohol.

We like being able to run both retorts at the same time, of course they
don't finish at the same time but closely. Your reagent usage drops also.

Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical Lab.
301-665-4980
fax 301-665-4941


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol Bryant
Sent: Wednesday, July 22, 2009 11:09 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Peloris tissue processor 

Anyone currently using the Peloris tissue processor please comment- love
it, likes, dislikes?

Thank you in advance for your input. 

 

Carol Bryant, CT (ASCP)

Cytology/Histology Manager

Pathology Services

Lexington Clinic

Phone (859) 258-4082

Fax (859) 258-4081

cbrya <@t> lexclin.com 

 

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