[Histonet] Fluorescent dyes and xylene

[Bob Nienhuis]

Will the Alexa Fluor dyes withstand some dehydration and clearing in
alcohol and xylene?

If they won't are there any bright fluorescent dyes that can be obtained
congugated to a secondary antibody that will?

Looking to immunostain tyrosine hydroxylase in rodent brain with
a fluorescent marker in tissue that needs to be dehydrated and
cleared, perhaps with an abbreviated series.

Bob Nienhuis
VA / UCLA Neurobiology Research