[Histonet] Fluorescent dyes and xylene

Bob Nienhuis bob.nienhuis <@t> gmail.com
Mon Jul 20 12:37:07 CDT 2009


Will the Alexa Fluor dyes withstand some dehydration and clearing in
alcohol and xylene?

If they won't are there any bright fluorescent dyes that can be obtained
congugated to a secondary antibody that will?

Looking to immunostain tyrosine hydroxylase in rodent brain with
a fluorescent marker in tissue that needs to be dehydrated and
cleared, perhaps with an abbreviated series.

Bob Nienhuis
VA / UCLA Neurobiology Research


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