[Histonet] nuclear bubbling

[Rene J Buesa]

Exactly! The sections cannot have any water remaining between them and the slide. That is the thing. Microwaving them in that condition (with water underneath) will also produce the same artifact.
René J.

--- On Fri, 7/17/09, Harrison, Sandra C. <Sandra.Harrison3 <@t> va.gov> wrote:


From: Harrison, Sandra C. <Sandra.Harrison3 <@t> va.gov>
Subject: RE: [Histonet] nuclear bubbling
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, July 17, 2009, 5:27 PM


How about microwaving to dry slides?  Can that cause nuclear bubbling?  

When you say completely drained off, does that mean your slides have to be completely dry prior to placing them in the oven?

Thanks,
Sandy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, July 15, 2009 2:59 PM
To: Histonet; Joyce Cline
Subject: Re: [Histonet] nuclear bubbling

Anybody can experience nuclear bubbling in any type tissue as long as the sections as set to dry at high temperature BEFORE they are completely drained off!
René J.

--- On Wed, 7/15/09, Joyce Cline <jcline <@t> wchsys.org> wrote:


From: Joyce Cline <jcline <@t> wchsys.org>
Subject: [Histonet] nuclear bubbling
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, July 15, 2009, 3:45 PM


Has anyone experienced nuclear bubbling on prostate biopsies? 



Joyce 





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