[Histonet] Fat histology

[Michelle MacVeigh-Aloni]

Hi Karen,

I had the same problem recently. 
I got very good advise trough the histonet for working with fat. You might want to check the arhives.

In my case, I was in a big time crunch with this dog fat, so after all the fat was collected and stored for a while in 10% NBF I did'nt have any time to post fix it, I just had to put it in the processor. It came out great! 
I used 1 hour in all steps and the steps are:

1    Formalin    1 hour
2    80%          1 hour
3    95%          1 hour
4    95%          1 hour
5    100%        1 hour
6    100%        1 hour
7    100 %       1 hour
8    Clear Rite  1 hour
9    Clear Rite  1 hour
10  Clear Rite  1 hour
11  Paraffin      1 hour
12  Paraffin      1 hour
13  Paraffin      1 hour
14  Paraffin      1 hour

I did it again few days ago (the same way) and again had great result. We also did IF on this fat and it worked great as well.

Good luck with it
Michelle

Research Specialist 
USC Keck School of Medicine
Los Angeles, CA