[Histonet] VonKossa's calcium stain

[Jack Ratliff]

What is your tissue of interest? Why not do the Von Kossa stain first  
and then counterstain with MacNeal's tetrachrome. This way you employ  
the use of a metachromatic stain for the rest of the tissue instead of  
just a nuclear staining hematoxylin.

Jack


On Jul 16, 2009, at 9:34 PM, "karine cadoret" <kcadoret <@t> amc.edu.au>  
wrote:

> Hi,
>
> When doing a VonKossa stain in order to demonstrate calcium in tissue,
> does it matter much if I use Mayer's hematoxylin instead of Ehrlich's
> hematoxylin (which takes 6 months to ripen) ?
>
> Also, can I simply use homemade scott's tapwater for blueing instead  
> of
> using a lithium carbonate solution ?
>
>
>
> Thank you for your help,
>
>
>
> Karine Cadoret
>
> Fish health laboratory manager
>
> National Center for Marine Conservation and Resource Sustainability
>
> Newnham, TAS
>
> Australia
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>