[Histonet] VonKossa's calcium stain

[karine cadoret]

Hi, 

When doing a VonKossa stain in order to demonstrate calcium in tissue,
does it matter much if I use Mayer's hematoxylin instead of Ehrlich's
hematoxylin (which takes 6 months to ripen) ? 

Also, can I simply use homemade scott's tapwater for blueing instead of
using a lithium carbonate solution ? 

 

Thank you for your help, 

 

Karine Cadoret

Fish health laboratory manager

National Center for Marine Conservation and Resource Sustainability

Newnham, TAS

Australia