[Histonet] IHC frozens

[Patti Loykasek]

Hi Kim. You can do HIER on frozen sections, but first you must fix the
sections. The purpose of HIER is to 'unmask' antigens that have been altered
by the effects of fixation. We have had better results with many antibodies
by fixing the frozen sections in 10% buffered formalin (30'-60'), then doing
a gentle heat retrieval (~70C) or using a mild enzyme. Of course, there are
times when it is preferable to use the frozen sections as is & determine if
the antibody will recognize the epitope in this 'native' state.
Just my experience.


Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA




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