[Histonet] Re: Leica Autostainer Problems

Asquith Stephen stephen.asquith <@t> petermac.org
Thu Jul 2 18:04:37 CDT 2009


Hi All
Many thanks for the posts that were sent regarding our problem. The
local Leica agents in John and Marina came out and checked out the
machine. The next day Marina spent the day with us trouble shooting with
a perfect H&E as an end result. The problem was sloved by including a
wash step prior to the dehydration steps after the Eosin. This ensured
all the eosin was removed from the slide prior to the dehydration steps.
Droplets of eosin were being left on the lable portion of the slides and
these then dripped down the slide removing some of the eosin stain and
hence our problem.
Happy histologists all round. Many thanks to Marina

Stephen Asquith

Microscopy Imaging and Research Core Facility
Peter MacCallum Cancer Centre
Locked Bag #1
A'Beckett Street
East Melbourne  8006

Email:  stephen.asquith <@t> petermac.org
Phone +61-3-9656 1244/1243
Fax +61-3-9656 1411




This email (including any attachments or links) may contain 
confidential and/or legally privileged information and is 
intended only to be read or used by the addressee.  If you 
are not the intended addressee, any use, distribution, 
disclosure or copying of this email is strictly 
prohibited.  
Confidentiality and legal privilege attached to this email 
(including any attachments) are not waived or lost by 
reason of its mistaken delivery to you.
If you have received this email in error, please delete it 
and notify us immediately by telephone or email.  Peter 
MacCallum Cancer Centre provides no guarantee that this 
transmission is free of virus or that it has not been 
intercepted or altered and will not be liable for any delay 
in its receipt.


More information about the Histonet mailing list