[Histonet] JB4 users

[Denise Crowley]

We are a research core Histology facility accepting specimens from  
within the MIT community.  One of our researchers is trying to embed  
mouse femurs in JB4 for us to section on our Thermo Finesse microtome  
utilizing a tungsten carbide blade.    The tissue just crumbles out  
of the resin and we are not able to pick up any sections.  We asked  
the researcher to infiltrate longer, which she did, but the results  
are the same.  We have sectioned other tissues in JB4 with this  
instrument, so I do not believe the problem is us or the microtome.   
Any suggestions would be appreciated.

Denise Crowley
Facility Manager Histology
David H. Koch Institute for Integrative Cancer Research
Massachusetts Institute of Technology
40 Ames St. E17-427
Cambridge MA  02139
617-258-8183
dencrowl <@t> mit.edu